Liu Huimin, Liu Bingjie, Zhang Siqi, Fan Miao, Ji Xiaoxue, Zhang Shouan, Wang Zhongtang, Qiao Kang
Key Laboratory of Pesticide Toxicology & Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Ecotoxicol Environ Saf. 2023 Oct 15;265:115510. doi: 10.1016/j.ecoenv.2023.115510. Epub 2023 Sep 22.
Fluopyram, a SDH inhibitor fungicide, is widely used in agriculture to control fungi and nematodes. However, fluopyram has been proved toxic that caused damage to organs through oxidative stress. The development of natural extracts that can reduce oxidative damage is a promising method. Lentinan is isolated from Lentinus edodes and has been verified its antioxidant activity. In this study, Caenorhabditis elegans was used to evaluate the protective effects of lentinan against fluopyram-induced toxicity and the possible mechanisms. Results showed that lentinan pretreatment notably increased the survival rate of N2 nematodes by 15.0 % and extended the lifespan by 91.5 %, compared with the fluopyram treatment. Lentinan pretreatment reverted the inhibition of the locomotion and reproduction of C. elegans under the fluopyram stress. In addition, lentinan pretreatment significantly decreased the contents of ROS and MDA in N2 nematodes. Moreover, pretreated with lentinan significantly recovered the decreased activities of CAT, SOD, GST and SDH induced by fluopyram. Lentinan pretreatment enhanced the mRNA levels of daf-16 and skn-1 and their downstream genes in the nematodes compared with the fluopyram group. In daf-16 and skn-1 mutants, the lifespan, ROS and related genes expression were not significantly changed in lentinan pretreatment. Pretreated with lentinan significantly enhanced the fluorescence intensity of SOD-3::GFP and GST-4::GFP, and promoted the nuclear translocation of DAF-16 and SKN-1 under the fluopyram stress. In summary, these findings indicated that lentinan protected C. elegans from fluopyram-induced toxicity via DAF-16 and SKN-1.
氟吡菌酰胺是一种琥珀酸脱氢酶（SDH）抑制剂类杀菌剂，广泛应用于农业领域以防治真菌和线虫。然而，氟吡菌酰胺已被证明具有毒性，可通过氧化应激对器官造成损害。开发能够减少氧化损伤的天然提取物是一种很有前景的方法。香菇多糖是从香菇中分离得到的，其抗氧化活性已得到验证。在本研究中，利用秀丽隐杆线虫评估香菇多糖对氟吡菌酰胺诱导毒性的保护作用及其可能的机制。结果表明，与氟吡菌酰胺处理组相比，香菇多糖预处理显著提高了N2线虫15.0%的存活率，并延长了91.5%的寿命。香菇多糖预处理逆转了氟吡菌酰胺胁迫下秀丽隐杆线虫运动和繁殖的抑制。此外，香菇多糖预处理显著降低了N2线虫中活性氧（ROS）和丙二醛（MDA）的含量。而且，香菇多糖预处理显著恢复了氟吡菌酰胺诱导降低的过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽S-转移酶（GST）和琥珀酸脱氢酶（SDH）的活性。与氟吡菌酰胺组相比，香菇多糖预处理提高了线虫中daf-16和skn-1及其下游基因的mRNA水平。在daf-16和skn-1突变体中，香菇多糖预处理对寿命、ROS及相关基因表达没有显著影响。在氟吡菌酰胺胁迫下，香菇多糖预处理显著增强了SOD-3::GFP和GST-4::GFP的荧光强度，并促进了DAF-16和SKN-1的核转位。综上所述，这些发现表明香菇多糖通过DAF-16和SKN-1保护秀丽隐杆线虫免受氟吡菌酰胺诱导的毒性。
