Institute of Biochemistry and Physiology of Plants and Microorganisms - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 410049 Saratov, Russia.
Front Biosci (Elite Ed). 2023 Jul 26;15(3):19. doi: 10.31083/j.fbe1503019.
Infection diagnosis and antibiotic sensitivity testing are important aspects of clinical microbiology that are in dire need of improvement owing to the inadequate current standards in the early detection of bacterial response to antibiotics. The increasing antimicrobial resistance is a serious global threat to human health. Current resistance-detecting methods, using the phenotypic antibiotic sensitivity test, which measures bacterial growth as affected by antibiotics, have long analysis times. Therefore, new and rapid methods are needed to detect antibiotic resistance. Here, we review the methods used to detect antibiotic resistance in bacteria, including that caused by biofilm development, and we look at the development of rapid methods for evaluating antimicrobial resistance (AMR).
感染诊断和抗生素敏感性测试是临床微生物学的重要方面，由于目前早期检测抗生素对细菌反应的标准不足，因此急需改进。日益增加的抗生素耐药性是对人类健康的严重全球威胁。目前的耐药性检测方法，使用表型抗生素敏感性测试，即测量抗生素对细菌生长的影响，其分析时间较长。因此，需要新的快速方法来检测抗生素耐药性。在这里，我们回顾了用于检测细菌（包括生物膜发展引起的细菌）抗生素耐药性的方法，并研究了评估抗菌药物耐药性（AMR）的快速方法的发展。
