Rollano-Peñaloza Oscar M, Mollinedo Patricia A, Widell Susanne, Rasmusson Allan G
Instituto de Investigaciones Quimicas, Universidad Mayor de San Andrés, La Paz, Bolivia.
Department of Biology, Lund University, Lund, Sweden.
Front Fungal Biol. 2021 Dec 1;2:768648. doi: 10.3389/ffunb.2021.768648. eCollection 2021.
Symbiotic strains of fungi in the genus affect growth and pathogen resistance of many plant species, but the interaction is not known in molecular detail. Here we describe the transcriptomic response of two cultivars of the crop to axenic co-cultivation with BOL-12 and T22. The response of roots to BOL-12 and T22 in the early phases of interaction was studied by RNA sequencing and RT-qPCR verification. Interaction with the two fungal strains induced partially overlapping gene expression responses. Comparing the two plant genotypes, a broad spectrum of putative quinoa defense genes were found activated in the cultivar Kurmi but not in the Real cultivar. In cultivar Kurmi, relatively small effects were observed for classical pathogen response pathways but instead a -specific clade of germin-like genes were activated. Germin-like genes were found to be more rapidly induced in cultivar Kurmi as compared to Real. The same germin-like genes were found to also be upregulated systemically in the leaves. No strong correlation was observed between any of the known hormone-mediated defense response pathways and any of the quinoa- interactions. The differences in responses are relevant for the capabilities of applying agents for crop protection of different cultivars of .
该属真菌的共生菌株会影响许多植物物种的生长和抗病性，但这种相互作用的分子细节尚不清楚。在此，我们描述了两种作物品种与BOL - 12和T22进行无菌共培养时的转录组反应。通过RNA测序和RT - qPCR验证研究了藜麦根系在相互作用早期对BOL - 12和T22的反应。与两种真菌菌株的相互作用诱导了部分重叠的基因表达反应。比较这两种植物基因型，发现广泛的假定藜麦防御基因在库尔米品种中被激活，而在里尔品种中未被激活。在库尔米品种中，经典病原体反应途径的影响相对较小，相反，一个特定的类萌发素基因分支被激活。与里尔品种相比，在库尔米品种中发现类萌发素基因诱导得更快。同样的类萌发素基因在叶片中也被系统性地上调。在任何已知的激素介导的防御反应途径与藜麦与真菌的任何相互作用之间未观察到强烈的相关性。这些反应差异对于应用真菌剂保护不同藜麦品种的能力具有重要意义。
