Miyazawa Ken, Yamashita Takaaki, Takeuchi Ayumu, Kamachi Yuka, Yoshimi Akira, Tashiro Yuto, Koizumi Ami, Ogata Makoto, Yano Shigekazu, Kasahara Shin, Sano Motoaki, Yamagata Youhei, Nakajima Tasuku, Abe Keietsu
Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Laboratory of Filamentous Mycoses, Department of Fungal Infection, National Institute of Infectious Diseases, Tokyo, Japan.
Front Fungal Biol. 2022 Jan 28;2:821946. doi: 10.3389/ffunb.2021.821946. eCollection 2021.
α-1,3-Glucan is one of the main polysaccharides in the cell wall of . We previously revealed that it plays a role in hyphal aggregation in liquid culture, and that its molecular mass (MM) in an -overexpressing () strain was larger than that in an -overexpressing () strain. The mechanism that regulates its MM is poorly understood. Although the gene , which encodes glycosylphosphatidylinositol (GPI)-anchored α-amylase (AmyD), is involved in the biosynthesis of α-1,3-glucan in . , how it regulates this biosynthesis remains unclear. Here we constructed strains with disrupted (Δ) or overexpressed () in the genetic background of the ABPU1 (wild-type), , or strain, and characterized the chemical structure of α-1,3-glucans in the cell wall of each strain, focusing on their MM. The MM of α-1,3-glucan from the strain was smaller than that in the parental strain. In addition, the MM of α-1,3-glucan from the Δ strain was greater than that in the strain. These results suggest that AmyD is involved in decreasing the MM of α-1,3-glucan. We also found that the C-terminal GPI-anchoring region is important for these functions.
α-1,3-葡聚糖是……细胞壁中的主要多糖之一。我们先前发现它在液体培养中参与菌丝聚集，并且在一个……过表达（……）菌株中其分子量（MM）大于在另一个……过表达（……）菌株中的分子量。调节其分子量的机制尚不清楚。尽管编码糖基磷脂酰肌醇（GPI）锚定的α-淀粉酶（AmyD）的基因……参与了……中α-1,3-葡聚糖的生物合成，但其如何调节这种生物合成仍不清楚。在这里，我们在ABPU1（野生型）、……或……菌株的遗传背景下构建了……缺失（Δ）或……过表达（……）的菌株，并对每个菌株细胞壁中α-1,3-葡聚糖的化学结构进行了表征，重点关注它们的分子量。来自……菌株的α-1,3-葡聚糖的分子量小于其亲本……菌株中的分子量。此外，来自……Δ菌株的α-1,3-葡聚糖的分子量大于……菌株中的分子量。这些结果表明AmyD参与降低α-1,3-葡聚糖的分子量。我们还发现C端GPI锚定区域对这些功能很重要。
