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秀丽隐杆线虫生殖细胞颗粒既影响生殖细胞又影响体细胞的 RNA 组。

C. elegans germ granules sculpt both germline and somatic RNAome.

机构信息

Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH, 43210, USA.

Center for RNA Biology, The Ohio State University, Columbus, OH, 43210, USA.

出版信息

Nat Commun. 2023 Sep 25;14(1):5965. doi: 10.1038/s41467-023-41556-4.

DOI:10.1038/s41467-023-41556-4
PMID:37749091
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10520050/
Abstract

Germ granules are membrane-less organelles essential for small RNA biogenesis and germline development. Among the conserved properties of germ granules is their association with the nuclear membrane. Recent studies demonstrated that LOTUS domain proteins, EGGD-1 and EGGD-2 (also known as MIP-1 and MIP-2 respectively), promote the formation of perinuclear germ granules in C. elegans. This finding presents a unique opportunity to evaluate the significance of perinuclear localization of germ granules. Here we show that loss of eggd-1 causes the coalescence of germ granules and formation of abnormal cytoplasmic aggregates. Impairment of perinuclear granules affects certain germline classes of small RNAs including Piwi-interacting RNAs. Transcriptome profiling reveals overexpression of spermatogenic and cuticle-related genes in eggd-1 hermaphrodites. We further demonstrate that disruption of germ granules activates HLH-30-mediated transcriptional program in somatic tissues. Collectively, our findings underscore the essential role of EGGD-1 in germ granule organization and reveal an unexpected germ granule-to-soma communication.

摘要

生殖质是一种无膜细胞器，对于小 RNA 的生物发生和生殖细胞的发育至关重要。生殖质的保守特性之一是与核膜的关联。最近的研究表明，LOTUS 结构域蛋白 EGGD-1 和 EGGD-2（也分别称为 MIP-1 和 MIP-2）促进了线虫中核周生殖质的形成。这一发现为评估生殖质核周定位的意义提供了一个独特的机会。在这里，我们发现 eggd-1 的缺失导致生殖质的合并和异常细胞质聚集体的形成。核周颗粒的损伤会影响包括 Piwi 相互作用 RNA 在内的某些生殖细胞类别的小 RNA。转录组谱分析显示，在 eggd-1 雌雄同体中，精细胞发生和角质层相关基因的表达过度。我们进一步证明，生殖质的破坏会激活 HLH-30 介导的体组织中的转录程序。总之，我们的研究结果强调了 EGGD-1 在生殖质组织中的重要作用，并揭示了一种意想不到的生殖质与体组织之间的通讯。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/6ffacbfca9c1/41467_2023_41556_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/149be9575759/41467_2023_41556_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/5a0c544e5662/41467_2023_41556_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/86a7b91a1684/41467_2023_41556_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/4e7f52b69f0e/41467_2023_41556_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/4ebf25ecbe58/41467_2023_41556_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/0d3cc9836e83/41467_2023_41556_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/6ffacbfca9c1/41467_2023_41556_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/149be9575759/41467_2023_41556_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/5a0c544e5662/41467_2023_41556_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/86a7b91a1684/41467_2023_41556_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/4e7f52b69f0e/41467_2023_41556_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/4ebf25ecbe58/41467_2023_41556_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/0d3cc9836e83/41467_2023_41556_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/10520050/6ffacbfca9c1/41467_2023_41556_Fig7_HTML.jpg

相似文献

[1]
C. elegans germ granules sculpt both germline and somatic RNAome.

Nat Commun. 2023-9-25

[2]
Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in .

Elife. 2021-11-3

[3]
Germ-granule components prevent somatic development in the C. elegans germline.

Curr Biol. 2014-5-5

[4]
Germ Granules Prevent Accumulation of Somatic Transcripts in the Adult Germline.

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[5]
Germ granules and gene regulation in the Caenorhabditis elegans germline.

Genetics. 2022-3-3

[6]
Caenorhabditis elegans germ granules are present in distinct configurations and assemble in a hierarchical manner.

Development. 2023-12-15

[7]
Novel LOTUS-domain proteins are organizational hubs that recruit Vasa to germ granules.

Elife. 2021-7-5

[8]
Connecting the Dots: Linking Caenorhabditis elegans Small RNA Pathways and Germ Granules.

Trends Cell Biol. 2021-5

[9]
ELLI-1, a novel germline protein, modulates RNAi activity and P-granule accumulation in Caenorhabditis elegans.

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[10]
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引用本文的文献

[1]
Nucleoporins shape germ granule architecture and balance small RNA silencing pathways.

Nat Commun. 2025-5-8

[2]
HLH-30/TFEB Rewires the Chaperone Network to Promote Proteostasis Upon Perturbations to the Coenzyme A and Iron-Sulfur Cluster Biosynthesis Pathways.

Aging Cell. 2025-6

[3]
Combinatorial tagging generates a multi-purpose knock-in mouse model revealing phase separation-dependent germ granules in RNA homeostasis and germline development.

Cell Death Differ. 2025-4-23

[4]
Crosstalk Between Phase-Separated Membraneless Condensates and Membrane-Bound Organelles in Cellular Function and Disease.

Adv Exp Med Biol. 2025

[5]
A nuclear pore-anchored condensate enables germ granule organization and transgenerational epigenetic inheritance.

Nat Struct Mol Biol. 2025-3-13

[6]
FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline.

Development. 2025-3-15

[7]
Genetically-Encoded Phase Separation Sensors Enable High-Fidelity Live-Cell Probing of Biomolecular Condensates.

ACS Sens. 2025-3-28

[8]
An AT-hook transcription factor promotes transcription of histone, spliced-leader, and piRNA clusters.

Nucleic Acids Res. 2025-2-8

[9]
Nucleoporins shape germ granule architecture and balance small RNA silencing pathways.

bioRxiv. 2025-1-24

[10]
Caenorhabditis Elegans as a Model for Environmental Epigenetics.

Curr Environ Health Rep. 2025-1-20

本文引用的文献

[1]
Nucleoporin foci are stress-sensitive condensates dispensable for C. elegans nuclear pore assembly.

EMBO J. 2023-7-3

[2]
Comparative analysis of piRNA sequences, targets and functions in nematodes.

RNA Biol. 2022-1

[3]
Biogenesis of spermatogenesis small RNAs is initiated by a zc3h12a-like ribonuclease.

Sci Adv. 2022-8-12

[4]
Imaging-associated stress causes divergent phase transitions of RNA-binding proteins in the Caenorhabditis elegans germ line.

G3 (Bethesda). 2022-8-25

[5]
Visualization and Purification of Germ Granule Proteins Using Proximity Labeling.

Bio Protoc. 2022-4-20

[6]
The conserved helicase ZNFX-1 memorializes silenced RNAs in perinuclear condensates.

Nat Cell Biol. 2022-7

[7]
The Caenorhabditis elegans TDRD5/7-like protein, LOTR-1, interacts with the helicase ZNFX-1 to balance epigenetic signals in the germline.

PLoS Genet. 2022-6

[8]
Germ granules and gene regulation in the Caenorhabditis elegans germline.

Genetics. 2022-3-3

[9]
piRNAs initiate transcriptional silencing of spermatogenic genes during C. elegans germline development.

Dev Cell. 2022-1-24

[10]
Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in .

Elife. 2021-11-3

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