基于小分子有机探针的亚 10nm 分辨率三维多色光学纳米显微镜技术。
Three-dimensional multi-color optical nanoscopy at sub-10-nm resolution based on small-molecule organic probes.
机构信息
College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China; Britton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China.
出版信息
Cell Rep Methods. 2023 Sep 25;3(9):100556. doi: 10.1016/j.crmeth.2023.100556. Epub 2023 Aug 14.
Achieving nanometer-scale resolution remains challenging in expansion microscopy due to photon loss. To address this concern, here we develop a multi-color expansion stimulated emission depletion technique based on small-molecule probes to realize high labeling density and intensity. Our method substantially lowers the barrier to visualizing diverse intracellular proteins and their interactions in three dimensions. It enables us to achieve sub-10-nm resolution in structures such as microfilaments, lysosomes, and mitochondria, providing new insights into cell biology.
在扩展显微镜中，由于光子损失，实现纳米级分辨率仍然具有挑战性。为了解决这个问题，我们在这里开发了一种基于小分子探针的多色扩展受激发射损耗技术，以实现高标记密度和强度。我们的方法大大降低了在三维空间中可视化各种细胞内蛋白质及其相互作用的难度。它使我们能够在微丝、溶酶体和线粒体等结构中实现小于 10nm 的分辨率，为细胞生物学提供了新的见解。
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