Ruijs G J, van der Waaij D
Scand J Infect Dis. 1986;18(5):469-75. doi: 10.3109/00365548609032367.
An animal model of whole gut irrigation (WGI) in the rat was developed to study the effects of the irrigation procedure on the gastrointestinal microflora. Irrigation was performed with as well as without neomycin (1,000 mg/l) in the irrigation fluid. At the end of the irrigation procedure the animals were intragastrically contaminated with 2 neomycin-resistant Enterobacteriaceae strains (i.e. an Escherichia coli and a Klebsiella pneumoniae) to study the intestinal colonization by these strains. Although both contaminating strains persisted longer in the animals treated with irrigation fluid with neomycin than in those treated with mere irrigation fluid, persistence was only significantly longer with the E. coli strain (p less than 0.025). It seems likely that the condition of the--mainly anaerobic--mucosa-associated microflora after WGI was responsible for this difference in duration of colonization by the contaminating E. coli strain.
为了研究全肠道灌洗（WGI）程序对胃肠道微生物群的影响，建立了大鼠全肠道灌洗动物模型。灌洗过程中，灌洗液中添加和不添加新霉素（1000mg/L）。在灌洗程序结束时，给动物胃内接种2株耐新霉素的肠杆菌科菌株（即大肠杆菌和肺炎克雷伯菌），以研究这些菌株在肠道的定植情况。虽然与只用灌洗液处理的动物相比，用含新霉素的灌洗液处理的动物体内两种污染菌株持续存在的时间更长，但只有大肠杆菌菌株的持续时间显著更长（p<0.025）。WGI后主要为厌氧的黏膜相关微生物群的状态似乎是造成污染的大肠杆菌菌株定植持续时间存在这种差异的原因。
