Wang Si, Xu Jing, Guo Yuanxu, Cai Yongsong, Zhu Wenhua, Meng Liesu, Jiang Congshan, Lu Shemin
Clinical Laboratory, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Institute of Molecular and Translational Medicine (IMTM), and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.
Bio Protoc. 2023 Sep 20;13(18):e4823. doi: 10.21769/BioProtoc.4823.
The transfection of microRNA (miRNA) mimics and inhibitors can lead to the gain and loss of intracellular miRNA function, helping us better understand the role of miRNA during gene expression regulation under specific physical conditions. Our previous research has confirmed the efficiency and convenience of using liposomes to transfect miRNA mimics or inhibitors. This work uses miR-424 as an example, to provide a detailed introduction for the transfection process of miRNA mimics and inhibitors in the regular SW982 cell line and primary rheumatoid arthritis synovial fibroblasts (RASF) cells from patients by using lipofection, which can also serve as a reference to miRNA transfection in other cell lines. Key features • MiRNA mimics and inhibitors transfection in regular SW982 cell line and primary RASF cells. • Treatment and culture of RASF primary cells before transfection. • Using liposomes for transfection purposes.
转染微小RNA（miRNA）模拟物和抑制剂可导致细胞内miRNA功能的获得和丧失，有助于我们更好地理解在特定生理条件下miRNA在基因表达调控过程中的作用。我们之前的研究已证实使用脂质体转染miRNA模拟物或抑制剂的有效性和便利性。本研究以miR-424为例，详细介绍了通过脂质转染法在常规SW982细胞系以及来自患者的原代类风湿性关节炎滑膜成纤维细胞（RASF）中进行miRNA模拟物和抑制剂转染的过程，这也可为其他细胞系中的miRNA转染提供参考。关键特性 • 在常规SW982细胞系和原代RASF细胞中进行miRNA模拟物和抑制剂转染。 • 转染前对RASF原代细胞进行处理和培养。 • 使用脂质体进行转染。
