Mehrabkhani Maryam, Movahhed Taraneh, Arefnezhad Mohsen, Hamedi Shokouhsadat, Faramarzian Farnaz
Department of Pediatric Dentistry, School of Dentistry, Mashhad University of Medical Sciences, Mashhad.
Kashmar School of Nursing, Mashhad University of Medical Sciences, Mashhad.
Eur J Transl Myol. 2023 Sep 22;33(4):11623. doi: 10.4081/ejtm.2023.11623.
This study aimed to evaluate the antimicrobial effect of hydro-alcoholic extract of apple (Malus domestica Borkh. Vs.golab, with and without ZnO nanoparticles) on Streptococcus Mutans bacterium compared to 0.2% Chlorhexidine, Persica and suspension of ZnO nanoparticles. Study samples were examined in the groups of apple hydro-alcoholic extract with and without addition of ZnO nanoparticles, a positive control group (Chlorhexidine 0.2%, Persica and suspension of ZnO nanoparticles), and a negative control group (distilled water). In this experiment, a concentration of 500 PPM of ZnO nanoparticles with a diameter of 0.4 nm was used. Agar diffusion method was used to determine the Minimum Inhibitory Concentration (MIC) of apple hydro-alcoholic extract with and without adding ZnO nanoparticles. The concentrations used were 200, 100, 50 and 25 mg/ml. ANOVA statistical test was used to compare the average in the study groups. According to our results, hydro-alcoholic extract of apples alone had no effect on the target bacteria in any of the concentrations. In the group of apple hydro-alcoholic extract with ZnO nanoparticles, the mean inhibition zone was 13 mm at a concentration of 25 mg/ml. 0.2% Chlorhexidine, Persica and suspension of ZnO nanoparticles was observed with the mean inhibition zone of 20 mm, 16 mm and 15 mm, respectively. Hydro-alcoholic extract of apple with addition of ZnO nanoparticles in concentration of 25mg/ml, had growth inhibitory effect on Streptococcus Mutans, but it was not remarkably efficient in comparison with Chlorhexidine.
本研究旨在评估苹果（金冠苹果，Malus domestica Borkh. Vs.golab，添加和未添加氧化锌纳米颗粒）水醇提取物对变形链球菌的抗菌效果，并与0.2%洗必泰、波斯菊提取物和氧化锌纳米颗粒悬浮液进行比较。研究样本分为添加和未添加氧化锌纳米颗粒的苹果水醇提取物组、阳性对照组（0.2%洗必泰、波斯菊提取物和氧化锌纳米颗粒悬浮液）和阴性对照组（蒸馏水）。在本实验中，使用了直径为0.4纳米、浓度为500 ppm的氧化锌纳米颗粒。采用琼脂扩散法测定添加和未添加氧化锌纳米颗粒的苹果水醇提取物的最低抑菌浓度（MIC）。使用的浓度分别为200、100、50和25毫克/毫升。采用方差分析统计检验比较各研究组的平均值。根据我们的结果，单独的苹果水醇提取物在任何浓度下对目标细菌均无作用。在添加氧化锌纳米颗粒的苹果水醇提取物组中，浓度为25毫克/毫升时平均抑菌圈为13毫米。观察到0.2%洗必泰、波斯菊提取物和氧化锌纳米颗粒悬浮液的平均抑菌圈分别为20毫米、16毫米和15毫米。添加浓度为25毫克/毫升氧化锌纳米颗粒的苹果水醇提取物对变形链球菌有生长抑制作用，但与洗必泰相比效果不显著。
