Hoffman Tammy R, Emsley Sarah A, Douglas Jenna C, Reed Kaela R, Esquivel Abigail R, Koyack Marc J, Paddock Brie E, Videau Patrick
Department of Biology, Southern Oregon University, Ashland, OR 97520, USA.
Department of Chemistry, Southern Oregon University, Ashland, OR 97520, USA.
BioTech (Basel). 2023 Sep 8;12(3):58. doi: 10.3390/biotech12030058.
While normal levels of reactive oxygen and nitrogen species (RONS) are required for proper organismal function, increased levels result in oxidative stress. Oxidative stress may be managed via the scavenging activities of antioxidants (e.g., curcumin) and the action of enzymes, including superoxide dismutase (SOD). In this work, the uptake and clearance of dietary curcuminoids (consisting of curcumin, demethoxycurcumin, and bisdemethoxycurcumin) was assessed in larvae following chronic or acute exposure. High levels of curcuminoid uptake and loss were observed within a few hours and leveled off within eight hours post treatment onset. The addition or removal of curcuminoids from media resulted in corresponding changes in SOD activity, and the involvement of each of the three genes was assessed for their contribution to total SOD activity. Taken together, these data provide insight into the uptake and clearance dynamics of curcuminoids and indicate that, while SOD activity generally increases following curcuminoid treatment, the individual genes appear to contribute differently to this response.
虽然正常水平的活性氧和氮物质（RONS）是机体正常功能所必需的，但水平升高会导致氧化应激。氧化应激可通过抗氧化剂（如姜黄素）的清除活性和包括超氧化物歧化酶（SOD）在内的酶的作用来控制。在这项研究中，评估了幼虫在长期或急性暴露后对膳食姜黄素类化合物（由姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素组成）的摄取和清除情况。在处理开始后的几个小时内观察到高水平的姜黄素类化合物摄取和损失，并在处理开始后八小时内趋于平稳。从培养基中添加或去除姜黄素类化合物会导致SOD活性发生相应变化，并评估了三个基因中每个基因对总SOD活性的贡献。综上所述，这些数据提供了对姜黄素类化合物摄取和清除动态的深入了解，并表明，虽然姜黄素类化合物处理后SOD活性通常会增加，但各个基因对这种反应的贡献似乎有所不同。
