Laboratory of Pharmacological Prospecting of Bioactive Products (BIOFARMATOX), Department of Antibiotics, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Department of Chemistry, Rural Federal University of Pernambuco, Av. Manuel de Medeiros, 96, Dois Irmãos, 52171-900, Recife, PE, Brazil.
Regul Toxicol Pharmacol. 2020 Jun;113:104643. doi: 10.1016/j.yrtph.2020.104643. Epub 2020 Mar 19.
Chrysobalanus icaco L. is a native plant of Brazil used as a food source and traditionally for the treatment of various diseases. The aim of study was performed the phytochemical analysis by UPLC-DAD-ESI-QTOF-MS/MS, and evaluated acute and repeated dose oral toxicities of the C. icaco L. leaf aqueous extract (AECi). The acute toxicity study was performed using a dose of AECi 2000 mg/kg, while the repeated dose toxicity study, the AECi was administered daily at doses of 100, 200 and 400 mg/kg, for 28 days. Behavior and mortality of animals were observed during the test period and body weight, as well water and eating consumption. Hematological, biochemical parameters and histopathological examinations were carried out. Phytochemical analysis of the AECi revealed the presence of flavonoids and tannins. Oral single dose of 2000 mg/kg of AECi resulted in no mortalities or abnormal clinical signs. Studies of repeated dose toxicity promoted a reduction in the body weight of treated animals and an increase of hepatic enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in both, males and females. Histopathological analyzes showed alterations in the livers of animals treated with AECi. Thus, this study recommends the population take care when using this species, especially during prolonged periods.
翅果藤（Chrysobalanus icaco L.）是一种原产于巴西的植物，被用作食物来源，并在传统上用于治疗各种疾病。本研究的目的是通过 UPLC-DAD-ESI-QTOF-MS/MS 进行植物化学分析，并评估翅果藤叶水提物（AECi）的急性和重复剂量口服毒性。急性毒性研究使用 AECi 2000 mg/kg 的剂量进行，而重复剂量毒性研究则每日以 100、200 和 400 mg/kg 的剂量给予 AECi，持续 28 天。在测试期间观察动物的行为和死亡率以及体重、水和食物消耗。进行了血液学、生化参数和组织病理学检查。AECi 的植物化学分析显示存在类黄酮和单宁。口服 AECi 单剂量 2000 mg/kg 不会导致死亡或出现异常临床症状。重复剂量毒性研究导致处理动物的体重减轻，并增加了雄性和雌性动物的丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）等肝酶。组织病理学分析显示，AECi 处理动物的肝脏发生了改变。因此，本研究建议人们在使用该物种时要注意，特别是在长时间使用时。
