Tao Lin, Tian Yingkuan, Liao Dengqing, An Songlin, Chen Wei, Liu Xiang, Xu Pei, Shen Xubo, Zhou Yuanzhong
Key Laboratory, School of Public Health, Zunyi Medical University, No.6 Xuefu West Road, Xinpu Street, Honghuagang District, Zunyi 563006, China.
Metabolites. 2023 Aug 29;13(9):978. doi: 10.3390/metabo13090978.
In this study, we evaluated the extraction effect of three different extractants, namely hexane + ether (/ = 3:1), acetonitrile and ethyl acetate, on polycyclic aromatic hydrocarbons (PAHs) and phthalic acid esters (PAEs) in placenta detected and analysed by triple quadrupole gas chromatography-mass spectrometry (GC-MS/MS). The results showed that n-hexane + ether (/ = 3:1) had the highest extraction efficiency. Under the optimal conditions, the limits of detection (LOD) for the 10 PAHs were 0.003-0.0167 μg/L with relative standard deviations (RSD) of 1.4-5.48% and detection rates of 68.19-107.05%, and the correlation coefficients were (R, 0.9982-0.9999). The LODs for the nine PAEs were 0.0015-3.5714 μg/L and the correlation coefficients were (R, 0.9982-0.9999). The limits of detection (S/N = 3) for the nine PAHs were 0.0015-0.5714 μg/L with relative standard deviations (RSD) of 3.15-8.37%, and the detection rates were 80.45-112.59% with correlations of (R, 0.9972-0.9998). The method was applied to the analysis of PAHs and phthalates in placenta samples from pregnant women. The method's accuracy and applicability were demonstrated. In comparison with other methods for the detection of PAEs and PAHs, the method proposed in this paper has a wider linear range, lower minimum detection limit and comparable recovery with good correlation. This paper is dedicated to providing another method for improving the performance of extracting solid tissues.
在本研究中，我们评估了三种不同萃取剂，即正己烷 + 乙醚（/ = 3:1）、乙腈和乙酸乙酯，对通过三重四极杆气相色谱 - 质谱联用仪（GC-MS/MS）检测和分析的胎盘中多环芳烃（PAHs）和邻苯二甲酸酯（PAEs）的萃取效果。结果表明，正己烷 + 乙醚（/ = 3:1）具有最高的萃取效率。在最佳条件下，10种多环芳烃的检测限（LOD）为0.003 - 0.0167 μg/L，相对标准偏差（RSD）为1.4 - 5.48%，检出率为68.19 - 107.05%，相关系数为（R，0.9982 - 0.9999）。9种邻苯二甲酸酯的检测限为0.0015 - 3.5714 μg/L，相关系数为（R，0.9982 - 0.9999）。9种多环芳烃的检测限（S/N = 3）为0.0015 - 0.5714 μg/L时，相对标准偏差（RSD）为3.15 - 8.37%，检出率为80.45 - 112.59%，相关性为（R，0.9972 - 0.9998）。该方法应用于孕妇胎盘样本中多环芳烃和邻苯二甲酸酯的分析，证明了该方法的准确性和适用性。与其他检测邻苯二甲酸酯和多环芳烃的方法相比，本文提出的方法具有更宽的线性范围、更低的最低检测限以及相当的回收率且相关性良好。本文致力于提供另一种提高固体组织萃取性能的方法。
