As a group of antibiotics largely used in China's animal husbandry, fluoroquinolone (FQ) residues in honey may pose potential threats to human health. This study performed a five-year investigation on the occurrence of FQ residues in honey in 521 and 160 honey samples collected from Zhejiang Province, China and compared FQ residue profiles in honey with a subgroup of various factors. Deterministic and probabilistic risk assessments of exposure to FQ residues in honey were further conducted. Overall, four FQs were detected in 6.9% (47/681) of analyzed samples; banned norfloxacin with the highest level (7890 μg·kg) and detection frequency (4.9%) was the primary safety risk factor associated with honeybees raised in China. FQ detection frequency and concentration of rape honey was highest among four of the largest and most stable honeys (rape, acacia, chaste, and linden) in China. Processed honey from commercial sale channels had a significantly higher detection frequency of FQ residues than raw honey from apiaries. Deterministic assessment showed that the noncarcinogenic hazard quotient (HQ) value of the dietary intake of FQs by the local population was between 4.75 × 10 and 1.18 × 10, less than 1.0, indicating that FQ residues in honey posed a low risk for consumers. The order of the HQ value was ciprofloxacin > norfloxacin > enrofloxacin > ofloxacin. Probabilistic assessment showed that at P95, the HQ of FQs for the age groups of children, adolescents, adults, and older adults over 65 years ranged from 2.39 × 10 to 0.217, less than 1, and the exposure risk for adults was higher than for children and adolescents. Sensitivity analysis showed that FQ concentrations were the major contributors to health risks. Although a low risk was found, a strict hive management is needed for beekeepers regarding troubles of food safety, international trade, and human bacterial resistance.