Food Chemistry Lab, Department of Chemistry and Technology of Drugs, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy.
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, Universitat de València, Avda Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
Toxins (Basel). 2023 Aug 31;15(9):538. doi: 10.3390/toxins15090538.
Beauvericin (BEA) is an emerging mycotoxin produced by some species of Fusarium genera that widely contaminates food and feed. is a protected medicinal plant known for its antioxidant and anti-inflammatory properties, which are attributed to its rich content of bioactive compounds. In order to evaluate the beneficial effects of flower against BEA cytotoxicity, the aim of this study is to evaluate changes in protein expression after Jurkat cell exposure through a proteomics approach. To carry out the experiment, cells were exposed to intestinally digested flower alone or in combination with the BEA standard (100 nM) over 7 days. Differentially expressed proteins were statistically evaluated ( < 0.05), revealing a total of 172 proteins with respect to the control in cells exposed to the BEA standard, 145 proteins for alone, and 139 proteins when exposing the cells to the combined exposure. Bioinformatic analysis revealed processes implicated in mitochondria, ATP-related activity, and RNA binding. After careful analysis of differentially expressed proteins, it was evident that attenuated, in most cases, the negative effects of BEA. Furthermore, it decreased the presence of major oncoproteins involved in the modulation of immune function.
beauvericin (BEA) 是一种由镰刀菌属真菌产生的新兴真菌毒素，广泛污染食物和饲料。 是一种受保护的药用植物，以其抗氧化和抗炎特性而闻名，这归因于其丰富的生物活性化合物含量。为了评估 花对 BEA 细胞毒性的有益作用，本研究旨在通过蛋白质组学方法评估 Jurkat 细胞暴露后蛋白质表达的变化。为了进行实验，将细胞单独或与 BEA 标准品（100 nM）一起在肠道消化 花后暴露 7 天。对差异表达蛋白进行了统计学评估（ < 0.05），结果显示，与对照组相比，暴露于 BEA 标准品的细胞中有 172 种蛋白，单独暴露于 花的细胞中有 145 种蛋白，而暴露于联合暴露的细胞中有 139 种蛋白。生物信息学分析显示，这些蛋白涉及线粒体、ATP 相关活性和 RNA 结合过程。对差异表达蛋白进行仔细分析后，发现 花在大多数情况下减轻了 BEA 的负面影响。此外，它降低了参与免疫功能调节的主要致癌蛋白的存在。
