Laboratory of Developmental Biology & Molecular Neurobiology, Department of Molecular Biology & Genetics, Democritus University of Thrace, GR-681 00 Alexandroupolis, Greece.
Laboratory of Molecular Regulation & Diagnostic Technology, Department of Molecular Biology & Genetics, Democritus University of Thrace, GR-681 00 Alexandroupolis, Greece.
Biomolecules. 2023 Aug 31;13(9):1337. doi: 10.3390/biom13091337.
Abracl (ABRA C-terminal-like protein) is a small, non-typical winged-helix protein that shares similarity with the C-terminal domain of the protein ABRA (Actin-Binding Rho-Activating protein). The role of Abracl in the cell remains elusive, although in cancer cells, it has been implicated in proliferation, migration and actin dynamics. Our previous study showed that mRNA was expressed in the dividing cells of the subpallial subventricular zone (SVZ), in the developing cortical plate (CP), and in the diencephalic SVZ; however, the molecular identities of the Abracl-expressing cell populations were not defined in that work. In this study, we use double immunofluorescence to characterize the expression of Abracl on sections of embryonic murine (E11.5-E18.5) and feline (E30/31-E33/34) telencephalon; to this end, we use a battery of well-known molecular markers of cycling (Ki67, Ascl1, Dlx2) or post-mitotic (Tubb3, Gad65/67, Lhx6 and Tbr1) cells. Our experiments show that Abracl protein has, compared to the mRNA, a broader expression domain, including, apart from proliferating cells of the subpallial and diencephalic SVZ, post-mitotic cells occupying the subpallial and pallial mantle (including the CP), as well as subpallial-derived migrating interneurons. Interestingly, in late embryonic developmental stages, Abracl was also transiently detected in major telencephalic fiber tracts.
Abracl（ABRA C 端样蛋白）是一种小的、非典型的翼状螺旋蛋白，与 ABRA（肌动蛋白结合的 Rho 激活蛋白）的 C 端结构域具有相似性。尽管在癌细胞中，Abracl 被牵连到增殖、迁移和肌动蛋白动力学中，但它在细胞中的作用仍然难以捉摸。我们之前的研究表明，mRNA 在副神经节下区（SVZ）的分裂细胞、皮质板（CP）和间脑 SVZ 中表达；然而，在之前的研究中，表达 Abracl 的细胞群体的分子特征尚未确定。在这项研究中，我们使用双重免疫荧光技术在胚胎鼠（E11.5-E18.5）和猫（E30/31-E33/34）端脑切片上对 Abracl 的表达进行了特征描述；为此，我们使用了一系列已知的细胞周期（Ki67、Ascl1、Dlx2）或有丝分裂后（Tubb3、Gad65/67、Lhx6 和 Tbr1）标志物。我们的实验表明，Abracl 蛋白的表达域比 mRNA 更广，除了 SVZ 下区和间脑的增殖细胞外，还包括占据 SVZ 下区和脑皮层帽的有丝分裂后细胞（包括 CP），以及源自 SVZ 的迁移中间神经元。有趣的是，在胚胎发育后期，Abracl 也在主要的端脑纤维束中短暂表达。
