Matias Margarida, Martins Alice, Alves Celso, Silva Joana, Pinteus Susete, Fitas Manuel, Pinto Pedro, Marto Joana, Ribeiro Helena, Murray Patrick, Pedrosa Rui
MARE-Marine and Environmental Sciences Centre and ARNET-Aquatic Research Network, Escola Superior de Turismo e Tecnologia do Mar, Polytechnic of Leiria, 2520-630 Peniche, Portugal.
LIFE-Health and Bioscience Research Institute, Technological University of Shannon, Moylish Park, V94 E8YF Limerick, Ireland.
Antioxidants (Basel). 2023 Aug 29;12(9):1684. doi: 10.3390/antiox12091684.
This work addresses the potential of the red seaweed as a source of bioactive ingredients for skin health and wellness in response to the growing awareness regarding the significance of sustainable strategies in developing new nature-based dermocosmetic products. Hydroalcoholic extracts from the dried biomass were subjected to sequential liquid-liquid partitions, affording five different fractions (F1-F5). Their cosmetic potential was assessed through a set of in vitro assays concerning their antioxidant, photoprotective, and healing properties. Additionally, their cytotoxicity in HaCaT cells and their capacity to induce inflammation in RAW 264.7 cells were also evaluated. As a proof-of-concept, O/W emulsions were prepared, and emulsion stability was assessed by optical microscopy, droplet size analysis, centrifugation tests, and rheology analysis. Furthermore, in vivo tests were conducted with the final formulation to assess its antioxidant capacity. At subtoxic concentrations, the most lipophilic fraction has provided photoprotection against UV light-induced photooxidation in HaCaT cells. This was conducted together with the aqueous fraction, which also displayed healing capacities. Regarding the physical and stability assays, the best performance was achieved with the formulation containing 1% aqueous extract, which exhibited water retention and antioxidant properties in the in vivo assay. In summary, displayed itself as a potential source of bioactive ingredients with multitarget properties for dermatological use.
随着人们越来越意识到可持续战略在开发新型天然源皮肤美容产品中的重要性，这项工作探讨了红海藻作为皮肤健康和保健生物活性成分来源的潜力。对干燥生物质的水醇提取物进行连续液-液分配，得到五个不同的馏分（F1-F5）。通过一系列关于其抗氧化、光保护和愈合特性的体外试验评估了它们的美容潜力。此外，还评估了它们在HaCaT细胞中的细胞毒性以及在RAW 264.7细胞中诱导炎症的能力。作为概念验证，制备了水包油乳液，并通过光学显微镜、液滴尺寸分析、离心试验和流变学分析评估了乳液稳定性。此外，对最终配方进行了体内试验以评估其抗氧化能力。在亚毒性浓度下，最亲脂性的馏分对HaCaT细胞中紫外线诱导的光氧化提供了光保护作用。这与水性馏分一起进行，水性馏分也显示出愈合能力。关于物理和稳定性试验，含有1%水提取物的配方表现最佳，在体内试验中表现出保水和抗氧化性能。总之，红海藻表现为一种具有多靶点特性的潜在生物活性成分来源，可用于皮肤病学。
