Phetruen Tanaporn, van Dam Bloem, Chanarat Sittinan
Laboratory of Molecular Cell Biology, Department of Biochemistry, Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Antioxidants (Basel). 2023 Sep 14;12(9):1765. doi: 10.3390/antiox12091765.
Andrographolide, a bioactive compound found in , has gained significant attention for its potential therapeutic properties. Despite its promising benefits, the understanding of its side effects and underlying mechanisms remains limited. Here, we investigated the impact of andrographolide in and observed that andrographolide induced cytotoxicity, particularly when oxidative phosphorylation was active. Furthermore, andrographolide affected various cellular processes, including vacuole fragmentation, endoplasmic reticulum stress, lipid droplet accumulation, reactive oxygen species levels, and compromised cell integrity. Moreover, we unexpectedly observed that andrographolide induced the precipitation of biomolecules secreted from yeast cells, adding an additional source of stress. Overall, this study provides insights into the cellular effects and potential mechanisms of andrographolide in yeast, shedding light on its side effects and underlying cytotoxicity pathways.
穿心莲内酯是一种存在于[具体来源未给出]中的生物活性化合物，因其潜在的治疗特性而受到广泛关注。尽管它有诸多益处，但对其副作用及潜在机制的了解仍然有限。在此，我们研究了穿心莲内酯对[具体对象未给出]的影响，观察到穿心莲内酯会诱导细胞毒性，尤其是在氧化磷酸化活跃时。此外，穿心莲内酯还影响多种细胞过程，包括液泡破碎、内质网应激、脂滴积累、活性氧水平以及细胞完整性受损。而且，我们意外地观察到穿心莲内酯会诱导酵母细胞分泌的生物分子沉淀，这增加了额外的应激源。总体而言，本研究深入探讨了穿心莲内酯在酵母中的细胞效应及潜在机制，揭示了其副作用及潜在的细胞毒性途径。
