Marfoglia Andrea, Tibourtine Fahd, Pilloux Ludovic, Cazalbou Sophie
CIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 31062 Toulouse, France.
Laboratoire de Génie Chimique, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 31062 Toulouse, France.
Bioengineering (Basel). 2023 Sep 5;10(9):1044. doi: 10.3390/bioengineering10091044.
Hydrogels (gels) are attractive tools for tissue engineering and regenerative medicine due to their potential for drug delivery and ECM-like composition. In this study, we use rheology to characterize GelMA/alginate gels loaded with human platelet lysate (PL). We then characterize these gels from a physicochemical perspective and evaluate their ability to transport PL proteins, their pore size, and their rate of degradation. Finally, their biocompatibility is evaluated. We describe how alginate changes the mechanical behavior of the gels from elastic to viscoelastic after ionic (calcium-mediated) crosslinking. In addition, we report the release of ~90% of PL proteins from the gels and relate it to the degradation profile of the gels. Finally, we evaluated the biocompatibility of the gels. Thus, the developed gels represent attractive substrates for both cell studies and as bioactive materials.
水凝胶因其在药物递送和类细胞外基质组成方面的潜力，成为组织工程和再生医学中颇具吸引力的工具。在本研究中，我们运用流变学来表征负载人血小板裂解液（PL）的甲基丙烯酰化明胶/海藻酸盐凝胶。接着，我们从物理化学角度对这些凝胶进行表征，并评估它们运输PL蛋白的能力、孔径大小以及降解速率。最后，评估它们的生物相容性。我们描述了离子（钙介导）交联后海藻酸盐如何使凝胶的力学行为从弹性转变为粘弹性。此外，我们报告了约90%的PL蛋白从凝胶中的释放情况，并将其与凝胶的降解情况相关联。最后，我们评估了凝胶的生物相容性。因此，所开发的凝胶对于细胞研究和作为生物活性材料而言，都是颇具吸引力的基质。
