Wu Shufang, Yin Jiaxin, Li Xuejuan, Xie Jingyi, Ding Hui, Han Lifeng, Bie Songtao, Li Fangyi, Zhu Beibei, Kang Liping, Song Xinbo, Yu Heshui, Li Zheng
College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Haihe Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Foods. 2023 Sep 6;12(18):3335. doi: 10.3390/foods12183335.
This work was designed to investigate the dynamic changes process of non-volatile organic compounds (n-VOCs) and volatile organic compounds (VOCs) in mulberries during different growth periods using UPLC-Q-Orbitrap-MS, HS-SPME-GC-MS, and HS-GC-IMS. A total of 166 compounds were identified, including 68 n-VOCs and 98 VOCs. Furthermore, principal component analysis (PCA), random forest analysis (RFA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze differences in mulberries at different ripening stages. A total of 74 compounds appeared or disappeared at different ripening periods and 24 compounds were presented throughout the growth process. Quantitative analysis and antioxidant experiments revealed that as the mulberries continued to mature, flavonoids and phenolic acids continued to increase, and the best antioxidant activity occurred from stage IV. Conclusively, an effective strategy was established for analyzing the composition change process during different growth periods, which could assist in achieving dynamic change process analysis and quality control.
本研究旨在利用超高效液相色谱-四极杆-轨道阱质谱联用仪（UPLC-Q-Orbitrap-MS）、顶空固相微萃取-气相色谱-质谱联用仪（HS-SPME-GC-MS）和顶空气相色谱-离子迁移谱仪（HS-GC-IMS），研究桑椹在不同生长时期非挥发性有机化合物（n-VOCs）和挥发性有机化合物（VOCs）的动态变化过程。共鉴定出166种化合物，其中包括68种n-VOCs和98种VOCs。此外，采用主成分分析（PCA）、随机森林分析（RFA）和正交偏最小二乘法判别分析（OPLS-DA）对不同成熟阶段的桑椹差异进行分析。共有74种化合物在不同成熟期出现或消失，24种化合物在整个生长过程中均有出现。定量分析和抗氧化实验表明，随着桑椹不断成熟，黄酮类化合物和酚酸含量持续增加，且在IV期抗氧化活性最佳。最终，建立了一种有效的策略来分析不同生长时期的成分变化过程，有助于实现动态变化过程分析和质量控制。
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