Ahlawat Sonika, Choudhary Vikas, Arora Reena, Kumar Ashish, Kaur Mandeep, Chhabra Pooja
ICAR-National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, India.
Department of Animal Husbandry and Dairying, Karnal 132001, Haryana, India.
Genes (Basel). 2023 Aug 22;14(9):1663. doi: 10.3390/genes14091663.
The molecular changes occurring in the host in response to in vivo parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across -infected and non-infected crossbred cows. The differential expression profiles from PBMCs of infected and non-infected crossbred cows were generated by RNA sequencing. A marked difference in the expression of genes associated with innate immunity (, , ) was observed between the two groups. The over-represented pathways in -infected cows were associated with the immune system and regulation of the mitotic cycle. Enriched genes and pathways in non-infected animals were associated with the maintenance of chromatin integrity and cell structure. The highly connected genes identified in this study form potential candidates for further investigation into host-parasite interactions in cattle. An improved understanding of the transcriptomic dynamics during theileriosis would lead to underpinning molecular level differences related to the health status of cattle.
宿主对体内寄生虫感染所发生的分子变化尚未得到充分了解。因此，本研究调查了感染和未感染杂交奶牛外周血单个核细胞（PBMCs）的差异表达谱。通过RNA测序生成了感染和未感染杂交奶牛PBMCs的差异表达谱。两组之间观察到与固有免疫相关基因（ 、 、 ）表达的显著差异。感染奶牛中过度表达的通路与免疫系统和有丝分裂周期调控有关。未感染动物中富集的基因和通路与染色质完整性和细胞结构的维持有关。本研究中鉴定出的高度连接基因构成了进一步研究牛宿主-寄生虫相互作用的潜在候选对象。更好地理解泰勒虫病期间的转录组动态变化将有助于确定与牛健康状况相关的分子水平差异。
