INDEGSAL, Cell Biology Area, Molecular Biology Department, Universidad de León, Campus de Vegazana s/n, 24071 León, Spain.
CNAG-CRG, Centre for Genomic Regulation, Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain.
Int J Mol Sci. 2023 Sep 14;24(18):14107. doi: 10.3390/ijms241814107.
Recent research has provided compelling evidence demonstrating that paternal exposure to different stressors can influence their offspring's phenotypes. We hypothesized that paternal stress can negatively impact the progeny, altering different miRs and triggering different physiological alterations that could compromise offspring development. To investigate this, we exposed zebrafish male siblings to a chronic stress protocol for 21 days. We performed RNA-sequencing (RNA-seq) analyses to identify differentially expressed small noncoding RNAs in 7-day postfertilization (dpf) larvae derived from paternally stressed males crossed with control females compared with the control progeny. We found a single miRNA differentially expressed-miR-29a-which was validated in larva and was also tested in the sperm, testicles, and brain of the stressed progenitors. We observed a vertical transmission of chronic stress to the unexposed larvae, reporting novel consequences of paternally inherited chronic stress at a molecular level. The deregulation of mi-R29a in those larvae could affect relevant biological processes affecting development, morphogenesis, or neurogenesis, among others. Additionally, these disruptions were associated with reduced rates of survival and hatching in the affected offspring.
最近的研究提供了令人信服的证据，表明父代暴露于不同的应激源会影响其后代的表型。我们假设父代应激会对后代产生负面影响，改变不同的 microRNA（miRs）并引发不同的生理变化，从而损害后代的发育。为了研究这一点，我们让雄性斑马鱼兄弟姐妹接受了 21 天的慢性应激方案。我们对来自父代应激雄性与对照雌性杂交的 7 天受精后（dpf）幼虫进行了 RNA 测序（RNA-seq）分析，以鉴定与对照后代相比差异表达的小非编码 RNA。我们发现了一个差异表达的 microRNA-miR-29a-在幼虫中得到了验证，并且在应激后代的精子、睾丸和大脑中也进行了测试。我们观察到慢性应激向未暴露的幼虫垂直传递，报告了父系遗传慢性应激在分子水平上的新后果。这些幼虫中 mi-R29a 的失调可能会影响发育、形态发生或神经发生等相关的生物学过程。此外，这些干扰与受影响后代的存活率和孵化率降低有关。
