State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
College of Life Sciences, Northeast Agricultural University, Harbin 150038, China.
Int J Mol Sci. 2023 Sep 16;24(18):14193. doi: 10.3390/ijms241814193.
The discovery and isolation of new non-Bt insecticidal bacteria and genes are significant for the development of new biopesticides against coleopteran pests. In this study, we evaluated the insecticidal activity of non-Bt insecticidal bacteria, PPBiotE33, IPPBiotC41, IPPBiotA42 and IPPBiotC43, isolated from the peanut rhizosphere. All these strains showed insecticidal activity against first- and third-instar larvae of , and . IPPBiotE33 showed the highest toxicity among the four strains and exhibited virulence against . The genome of IPPBiotE33 was sequenced, and a new protein, 03673, with growth inhibition effects on was obtained. In addition, IPPBiotE33 had a synergistic effect with Bt185 against in bioassays and back-inoculation experiments with peanut seedlings. IPPBiotE33 induced a decrease in hemocytes and an increase in phenol oxidase activity in hemolymph, known as the immunosuppressive effect, which mediated synergistic activity with Bt185. This study increased our knowledge of the new insecticidal strain IPPBiotE33 and shed new light on the research on new insecticidal coaction mechanisms and new blended pesticides.
从花生根际中分离到的非 Bt 杀虫细菌 PPBiotE33、IPPBiotC41、IPPBiotA42 和 IPPBiotC43 对鞘翅目害虫具有杀虫活性。这 4 株细菌对 、 和 1-3 龄幼虫均具有杀虫活性，其中 IPPBiotE33 对 3 龄幼虫的毒力最高。对 IPPBiotE33 进行基因组测序，获得一个新的蛋白 03673，该蛋白对 具有生长抑制作用。此外，在生物测定和花生幼苗回接实验中，IPPBiotE33 与 185 菌株 Bt 对 表现出增效作用。IPPBiotE33 诱导 血细胞减少和酚氧化酶活性增加，即免疫抑制作用，介导了与 Bt185 的增效作用。本研究增加了我们对新杀虫菌株 IPPBiotE33 的认识，为新杀虫协同作用机制和新型混配农药的研究提供了新的思路。
