Sun Kyung Hoon, Choi Cheol Hee, Cho Gwang-Won, Jang Chul Ho
Department of Emergency Medicine, College of Medicine, Chosun University, Gwangju 61452, Republic of Korea.
Department of Pharmacology, College of Medicine, Chosun University, Gwangju 61452, Republic of Korea.
J Pers Med. 2023 Aug 27;13(9):1317. doi: 10.3390/jpm13091317.
The impact of metformin on the rat facial nerve following crush injury has only occasionally been documented to date. The purpose of the current investigation was to use functional and electrophysiological evaluations to investigate the effects of metformin administration on recovery following crush injury to the rat facial nerve. The rats were randomly divided into four groups: the nonDM/PBS group (n = 4), the nonDM/metformin group (n = 4), the DM/PBS group (n = 4), and the DM/metformin group (n = 4). Diabetes was generated by an intraperitoneal injection of streptozotocin. Facial nerve paralysis was induced by a crush injury 7 days after diabetes induction. The blood glucose levels of the DM/PBS and DM/metformin groups were maintained at over 300 mg/dL, whereas the blood glucose levels of the nonDM/PBS and nonDM/metformin groups were maintained at less than 150 mg/dL. There was no significant difference between the two nonDM groups. In comparison to the PBS group, the metformin group's recurrence of vibrissa fibrillation occurred noticeably sooner over time. The nonDM/metformin group showed the highest recovery rate in the second, third, and fourth weeks post-crush, respectively. The threshold of action potential 4 weeks after crush injury showed that the nonDM/metformin group had a significantly lower mean threshold of MAP compared to other groups. The short-term effect of metformin on the recovery of facial nerve blood flow (FNBF) was significantly increased compared to the DM/PBS group. However, there was no significant difference in FNBF between the nonDM/metformin and nonDM/PBS groups. A diabetic condition promoted a delay in FN regeneration. Metformin is able to accelerate functional recovery in diabetic or nondiabetic FN-injured rats. Further studies using a morphometric or molecular approach are planned to understand the pharmacologic mechanism of metformin.
迄今为止，二甲双胍对大鼠面神经挤压伤的影响仅有偶尔的记录。本研究的目的是通过功能和电生理评估来研究二甲双胍给药对大鼠面神经挤压伤后恢复的影响。将大鼠随机分为四组：非糖尿病/磷酸盐缓冲液组（n = 4）、非糖尿病/二甲双胍组（n = 4）、糖尿病/磷酸盐缓冲液组（n = 4）和糖尿病/二甲双胍组（n = 4）。通过腹腔注射链脲佐菌素诱导糖尿病。在诱导糖尿病7天后，通过挤压伤诱导面神经麻痹。糖尿病/磷酸盐缓冲液组和糖尿病/二甲双胍组的血糖水平维持在300 mg/dL以上，而非糖尿病/磷酸盐缓冲液组和非糖尿病/二甲双胍组的血糖水平维持在150 mg/dL以下。两个非糖尿病组之间没有显著差异。与磷酸盐缓冲液组相比，二甲双胍组触须颤动的复发随着时间的推移明显更早出现。非糖尿病/二甲双胍组在挤压伤后的第二、第三和第四周分别显示出最高的恢复率。挤压伤4周后的动作电位阈值显示，与其他组相比，非糖尿病/二甲双胍组的平均动作电位阈值显著更低。与糖尿病/磷酸盐缓冲液组相比，二甲双胍对面神经血流（FNBF）恢复的短期影响显著增加。然而，非糖尿病/二甲双胍组和非糖尿病/磷酸盐缓冲液组之间的FNBF没有显著差异。糖尿病状态会促进FN再生延迟。二甲双胍能够加速糖尿病或非糖尿病FN损伤大鼠的功能恢复。计划进一步采用形态计量学或分子方法进行研究，以了解二甲双胍的药理机制。
