Kałucka Małgorzata, Podsadni Piotr, Szczepańska Agnieszka, Malinowska Eliza, Błażewicz Anna, Turło Jadwiga
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, 61 Żwirki i Wigury Street, 02-091 Warsaw, Poland.
Department of Pathobiochemistry and Interdisciplinary Applications of Ion Chromatography, Medical University of Lublin, 19 Chodźki Street, 20-093 Lublin, Poland.
Molecules. 2025 Jul 18;30(14):3015. doi: 10.3390/molecules30143015.
Our attempts to obtain a new mushroom-derived immunostimulatory preparation containing organically bound selenium and zinc have focused on the interactions between selenites and zinc(II) in liquid culture media and their effects on transport into the mushroom cell. Previously, we found that, even if Zn and SeO concentrations in the liquid medium are not high enough to precipitate ZnSeO, the accumulation of selenium in the presence of zinc, and zinc in the presence of selenites, significantly dropped. This effect was more dependent on the molar ratio of ions in the medium than on the concentration values. We hypothesized that the formation of zinc-selenite soluble complexes with charges depending on the ion concentration ratio in the aquatic environment affects the first stage of ion transport into the fungal cell-biosorption. To verify this, we found the zinc-selenite molar ratio at which the complexes of the highest stability are formed, examined the influence of the molar ratio of ions in the medium on the concentration of Zn and Se in the mushroom cell wall, and investigated the correlation between the concentration of selenites not bound in complex compounds and the Se concentration in the cell wall. The results indicate that the molar fraction of Zn(II) in a liquid medium in the range of 0.5-0.6 promotes the formation of the most stable complexes. At the same time, it significantly reduces the percentage of free selenites in the medium and most strongly inhibits the biosorption process of both zinc and selenium.
我们致力于获取一种新的源自蘑菇的免疫刺激制剂，该制剂含有有机结合的硒和锌，重点关注了亚硒酸盐与锌（II）在液体培养基中的相互作用及其对进入蘑菇细胞的转运的影响。此前，我们发现，即使液体培养基中的锌和亚硒酸盐浓度不足以使硒酸锌沉淀，在有锌存在时硒的积累以及在有亚硒酸盐存在时锌的积累也会显著下降。这种效应更多地取决于培养基中离子的摩尔比，而非浓度值。我们推测，在水环境中形成的带电荷的亚硒酸锌可溶性络合物，其电荷取决于离子浓度比，会影响离子转运进入真菌细胞的第一阶段——生物吸附。为了验证这一点，我们确定了形成最高稳定性络合物时的亚硒酸锌摩尔比，研究了培养基中离子摩尔比对蘑菇细胞壁中锌和硒浓度的影响，并探究了未结合在络合物中的亚硒酸盐浓度与细胞壁中硒浓度之间的相关性。结果表明，液体培养基中锌（II）的摩尔分数在0.5 - 0.6范围内会促进形成最稳定的络合物。与此同时，它会显著降低培养基中游离亚硒酸盐的百分比，并最强烈地抑制锌和硒的生物吸附过程。
