Alves-Silva Jorge Miguel, Pedreiro Sónia, Cruz Maria Teresa, Salgueiro Lígia, Figueirinha Artur
Univ Coimbra, Institute for Clinical and Biomedical Research, Health Sciences Campus, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal.
Univ Coimbra, Faculty of Pharmacy, Health Sciences Campus, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal.
Pharmaceuticals (Basel). 2023 Aug 23;16(9):1202. doi: 10.3390/ph16091202.
Inflammation plays a pivotal role in the resolution of infection or tissue damage. In addition, inflammation is considered a hallmark of aging, which in turn compromises wound healing. is an aromatic plant, whose infusion is traditionally used as an anti-inflammatory and wound-healing agent. In this study, a infusion was prepared and characterized by HPLC-PDA-ESI-MSn and its safety profile determined by the resazurin metabolic assay. The anti-inflammatory potential was revealed in lipopolysaccharide (LPS)-stimulated macrophages by assessing nitric oxide (NO) release and levels of inducible nitric oxide synthase (iNOS) and the interleukin-1β pro-form (pro-IL-1β). Wound-healing capacity was determined using the scratch assay. The activity of senescence-associated β-galactosidase was used to unveil the anti-senescent potential, along with the nuclear accumulation of yH2AX and p21 levels. The antiradical potential was assessed by DPPH and ABTS scavenging assays. The infusion contains predominantly rosmarinic acid and salvianolic acids. The extract decreased NO, iNOS, and pro-IL-1β levels. Interestingly, the extract promoted wound healing and decreased β-galactosidase activity, as well as yH2AX and p21 levels. The present work highlights strong antiradical, anti-inflammatory, and wound healing capacities, corroborating the traditional uses ascribed to this plant. We have described, for the first time for this extract, anti-senescent properties.
炎症在感染或组织损伤的消退过程中起关键作用。此外，炎症被认为是衰老的一个标志，而衰老反过来又会损害伤口愈合。[植物名称]是一种芳香植物，其浸剂传统上被用作抗炎和伤口愈合剂。在本研究中，制备了[植物名称]浸剂，并通过HPLC-PDA-ESI-MSn对其进行表征，同时通过刃天青代谢试验确定其安全性。通过评估一氧化氮（NO）释放、诱导型一氧化氮合酶（iNOS）和白细胞介素-1β前体（pro-IL-1β）水平，揭示了其在脂多糖（LPS）刺激的巨噬细胞中的抗炎潜力。使用划痕试验测定伤口愈合能力。衰老相关β-半乳糖苷酶的活性用于揭示其抗衰老潜力，同时检测γH2AX的核积累和p21水平。通过DPPH和ABTS清除试验评估其抗自由基潜力。该浸剂主要含有迷迭香酸和丹酚酸。提取物降低了NO、iNOS和pro-IL-1β水平。有趣的是，提取物促进了伤口愈合，降低了β-半乳糖苷酶活性以及γH2AX和p21水平。本研究突出了其强大的抗自由基、抗炎和伤口愈合能力，证实了该植物的传统用途。我们首次描述了该提取物的抗衰老特性。
