Alves-Silva Jorge M, Moreira Patrícia, Cavaleiro Carlos, Pereira Cláudia, Cruz Maria Teresa, Salgueiro Lígia
Univ Coimbra, Institute for Clinical and Biomedical Research, Health Sciences Campus, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal.
Univ Coimbra, Faculty of Pharmacy, Health Sciences Campus, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal.
Plants (Basel). 2023 Oct 31;12(21):3741. doi: 10.3390/plants12213741.
With the increase in global life expectancy, maintaining health into old age becomes a challenge, and research has thus concentrated on various strategies which aimed to mitigate the effects of skin aging. Aromatic plants stand out as promising sources of anti-aging compounds due to their secondary metabolites, particularly essential oils (EOs). The aim of this study was to ascribe to EO several biological activities that could be useful in the context of skin aging. The EO was obtained using hydrodistillation and characterized by gas chromatography-mass spectrometry (GC/MS). The anti-inflammatory potential was assessed using lipopolysaccharide (LPS)-stimulated macrophages. The effect on cell migration was disclosed using scratch wound assay. Lipogenesis was induced using T0901317, hyperpigmentation with 3-isobutyl-1-methylxantine (IBMX) and senescence with etoposide. Our results show that the EO was characterized mainly by α-pinene and limonene. The EO was able to decrease nitric oxide (NO) release as well as iNOS and pro-IL-1β protein levels. The EO promoted wound healing while decreasing lipogenesis and having depigmenting effects. The EO also reduced senescence-associated β-galactosidase, p21/p53 protein levels and the nuclear accumulation of γH2AX. Overall, our study highlights the properties of EO that make it a compelling candidate for dermocosmetics applications.
随着全球预期寿命的增加，维持老年时期的健康成为一项挑战，因此研究集中在各种旨在减轻皮肤衰老影响的策略上。芳香植物因其次生代谢产物，特别是精油（EOs），而成为有前景的抗衰老化合物来源。本研究的目的是赋予EO几种在皮肤衰老背景下可能有用的生物活性。EO通过水蒸馏法获得，并通过气相色谱-质谱联用（GC/MS）进行表征。使用脂多糖（LPS）刺激的巨噬细胞评估其抗炎潜力。使用划痕试验揭示其对细胞迁移的影响。使用T0901317诱导脂肪生成，使用3-异丁基-1-甲基黄嘌呤（IBMX）诱导色素沉着，使用依托泊苷诱导衰老。我们的结果表明，EO的主要特征是α-蒎烯和柠檬烯。EO能够减少一氧化氮（NO）释放以及诱导型一氧化氮合酶（iNOS）和前白细胞介素-1β（pro-IL-1β）蛋白水平。EO促进伤口愈合，同时减少脂肪生成并具有脱色作用。EO还降低了衰老相关的β-半乳糖苷酶、p21/p53蛋白水平以及γH2AX的核积累。总体而言，我们的研究突出了EO的特性，使其成为皮肤化妆品应用的有力候选者。
