Peng Bo-Rong, Zheng Li-Guo, Chen Lo-Yun, El-Shazly Mohamed, Hwang Tsong-Long, Su Jui-Hsin, Lee Mei-Hsien, Lai Kuei-Hung, Sung Ping-Jyun
Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei 110301, Taiwan.
Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung 804201, Taiwan.
Pharmaceuticals (Basel). 2023 Sep 6;16(9):1258. doi: 10.3390/ph16091258.
The marine sponge sp., collected from the Southern waters of Taiwan, was subjected to chemical composition screening, resulting in the isolation of four new 24-homoscalarane compounds, namely lendenfeldaranes R-U (-). The structures and relative stereochemistry of the new metabolites - were assigned based on NMR studies. The absolute configurations of compounds - were determined by comparing the calculated and experimental values of specific optical rotation. The antioxidant and anti-inflammatory activities of the isolated compounds were assayed using superoxide anion generation and elastase release assays. These assays are used to determine neutrophilic inflammatory responses of respiratory burst and degranulation. Compounds and inhibited superoxide anion generation by human neutrophils in response to formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B (fMLP/CB) with IC: 3.98-4.46 μM. Compounds and inhibited fMLP/CB-induced elastase release, with IC values ranging from 4.73 to 5.24 μM. These findings suggested that these new 24-homoscalarane compounds possess unique structures and potential anti-inflammatory activity.
从台湾南部海域采集的海洋海绵物种进行了化学成分筛选，从中分离出四种新的24-高海松烷类化合物，即lendenfeldaranes R-U（-）。新代谢产物的结构和相对立体化学通过核磁共振研究确定。通过比较比旋光度的计算值和实验值确定了化合物的绝对构型。使用超氧阴离子生成和弹性蛋白酶释放试验测定了分离化合物的抗氧化和抗炎活性。这些试验用于确定呼吸爆发和脱颗粒的嗜中性粒细胞炎症反应。化合物 和 抑制人中性粒细胞对甲酰-L-甲硫氨酰-L-亮氨酰-L-苯丙氨酸/细胞松弛素B（fMLP/CB）产生超氧阴离子，IC：3.98-4.46 μM。化合物 和 抑制fMLP/CB诱导的弹性蛋白酶释放，IC值范围为4.73至5.24 μM。这些发现表明，这些新的24-高海松烷类化合物具有独特的结构和潜在的抗炎活性。
