Lv Miaomiao, Hou Dejia, Wan Jiale, Ye Taozhi, Zhang Lin, Fan Jiangbo, Li Chunliu, Dong Yilun, Chen Wenqian, Rong Songhao, Sun Yihao, Xu Jinghong, Cai Liangjun, Gao Xiaoling, Zhu Jianqing, Huang Zhengjian, Xu Zhengjun, Li Lihua
Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China.
College of Engineering, Huazhong Agricultural University, Wuhan 430070, China.
Plants (Basel). 2023 Sep 21;12(18):3338. doi: 10.3390/plants12183338.
Drought stress is one of the major causes of crop losses. The WRKY families play important roles in the regulation of many plant processes, including drought stress response. However, the function of individual WRKY genes in plants is still under investigation. Here, we identified a new member of the WRKY families, , and analyzed its role in stress resistance by using a series of transgenic plant lines. positively regulates drought tolerance in rice. was expressed in all examined tissues and could be induced by various abiotic stresses and abscisic acid (ABA). was localized to the nucleus. Various abiotic stress-related cis-acting elements were observed in the promoters of . The results of -overexpressing plant analyses revealed that plays a positive role in drought stress tolerance. In addition, physiological analyses revealed that improves drought stress tolerance by improving the osmotic adjustment ability, oxidative stress tolerance, and water retention capacity of the plant. Furthermore, -overexpressing plants also showed higher sensitivity to exogenous ABA compared with that of wild-type rice (WT). Overexpression of also affected the transcript levels of ABA-responsive genes and the accumulation of ABA. These results indicate that plays a crucial role in the response to drought stress and may possess high potential value in improving drought tolerance in rice.
干旱胁迫是造成作物损失的主要原因之一。WRKY家族在调控包括干旱胁迫响应在内的许多植物生理过程中发挥着重要作用。然而，单个WRKY基因在植物中的功能仍在研究之中。在此，我们鉴定出WRKY家族的一个新成员，并通过一系列转基因株系分析了其在抗逆性中的作用。该基因正向调控水稻的耐旱性。它在所有检测的组织中均有表达，并且可被多种非生物胁迫和脱落酸（ABA）诱导。该基因定位于细胞核。在其启动子中观察到各种与非生物胁迫相关的顺式作用元件。过表达该基因的植株分析结果表明，它在干旱胁迫耐受性方面发挥着正向作用。此外，生理分析表明，该基因通过提高植物的渗透调节能力、氧化胁迫耐受性和保水能力来增强干旱胁迫耐受性。此外，与野生型水稻（WT）相比，过表达该基因的植株对外源ABA也表现出更高的敏感性。该基因的过表达还影响了ABA响应基因的转录水平以及ABA的积累。这些结果表明，该基因在干旱胁迫响应中起着关键作用，并且在提高水稻耐旱性方面可能具有很高的潜在价值。
