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干旱和外源脱落酸改变过氧化氢的积累,并差异调控两个玉米 RD22 类似基因的表达。

Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes.

机构信息

Plant Biotechnology Research Group, Department of Biotechnology, Life Sciences Building, University of the Western Cape, Robert Sobukwe Road, Bellville, 7530, South Africa.

DST-NRF Centre of Excellence in Food Security, University of the Western Cape, Robert Sobukwe Road, Bellville, 7530, South Africa.

出版信息

Sci Rep. 2017 Aug 18;7(1):8821. doi: 10.1038/s41598-017-08976-x.

Abstract

Increased biosynthesis of abscisic acid (ABA) occurs in plants in response to water deficit, which is mediated by changes in the levels of reactive oxygen species such as HO. Water deficit and ABA induce expression of some RD22-like proteins. This study aimed to evaluate the effect of water deficit and exogenous ABA (50 µM ABA applied every 24 hours for a total of 72 hours) on HO content in Zea mays (maize) and to characterise genes encoding two putative maize RD22-like proteins (designated ZmRD22A and ZmRD22B). The expression profiles of the two putative maize RD22-like genes in response to water deficit and treatment with ABA were examined in leaves. In silico analyses showed that the maize RD22-like proteins share domain organisation with previously characterized RD22-like proteins. Both water deficit and exogenous ABA resulted in increased HO content in leaves but the increase was more pronounced in response to water deficit than to exogenous ABA. Lignin content was not affected by exogenous ABA, whereas it was decreased by water deficit. Expression of both RD22-like genes was up-regulated by drought but the ZmRD22A gene was not influenced by exogenous ABA, whereas ZmRD22B was highly responsive to exogenous ABA.

摘要

在应对水分胁迫时,植物中脱落酸(ABA)的生物合成增加,这是由活性氧(如 HO)水平的变化介导的。水分胁迫和 ABA 诱导一些 RD22 样蛋白的表达。本研究旨在评估水分胁迫和外源 ABA(每隔 24 小时施加 50μM ABA,共 72 小时)对玉米中 HO 含量的影响,并对编码两个假定的玉米 RD22 样蛋白的基因(命名为 ZmRD22A 和 ZmRD22B)进行特征描述。在叶片中检测了两个假定的玉米 RD22 样基因对水分胁迫和 ABA 处理的表达谱。计算机分析表明,玉米 RD22 样蛋白与先前鉴定的 RD22 样蛋白具有相似的结构域组织。水分胁迫和外源 ABA 均导致叶片中 HO 含量增加,但水分胁迫引起的增加比外源 ABA 更为明显。外源 ABA 对木质素含量没有影响,而水分胁迫则降低了木质素含量。干旱胁迫上调了两个 RD22 样基因的表达,但 ZmRD22A 基因不受外源 ABA 的影响,而 ZmRD22B 对外源 ABA 高度响应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beaf/5562893/7755ca0a6460/41598_2017_8976_Fig1_HTML.jpg

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