Zhang Xianjiao, Feng Guang-Da, Liu Yang, Yang Fan, Li Yanxuan, Deng Xiaoqin, Zhu Honghui, Yao Qing
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Horticulture, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People's Republic of China.
Arch Microbiol. 2023 Sep 28;205(10):345. doi: 10.1007/s00203-023-03685-8.
Four Gram-staining-negative, aerobic, yellow-pigmented and rod-shaped bacteria, named strains BD1B2-1, NT2B1, YF14B1 and DM2B3-1, were isolated from four rhizosphere soil samples of banana in China. Comparison of the 16S rRNA gene sequences showed that all these strains were most closely related to an invalidly published species, 'Rhodocytophaga rosea' 172606-1, with similarities ranging from 87.7 to 88.0%. According to the phylogenomic analysis, the four strains were clustered in an independent lineage and closely related to the genus Rhodocytophaga. The genomic sizes of these strains were approximately 9.49-9.77 Mbp with the DNA G + C contents of 38.8-39.0 mol%. They all contained C ω5c, iso-C and iso-C 3-OH as the major fatty acids and menaquinone 7 as the only respiratory quinone. They all had phosphatidylethanolamine as the major polar lipids. Based on phenotypic and phylogenomic characteristics, the four strains should represent two novel species within a novel genus, for which the names Xanthocytophaga agilis gen. nov., sp. nov. (BD1B2-1 = GDMCC 1.2890 = JCM 35374) and Xanthocytophaga flavus sp. nov. (NT2B1 = GDMCC 1.2889 = JCM 35375) are proposed; the former is assigned as the type species of the novel genus Xanthocytophaga gen. nov. In addition, based on the phenotypic and phylogenomic data, we proposed to reclassify the existing genus Rhodocytophaga in the family Cytophagaceae into a novel family Rhodocytophagaceae fam. nov. The novel family consists of the type genus Rhodocytophaga and the novel genus Xanthocytophaga.
从中国香蕉的四个根际土壤样本中分离出四株革兰氏染色阴性、需氧、产黄色色素的杆状细菌，命名为BD1B2-1、NT2B1、YF14B1和DM2B3-1菌株。16S rRNA基因序列比较表明，所有这些菌株与一个未有效发表的物种“玫瑰红噬细胞菌”172606-1关系最为密切，相似度在87.7%至88.0%之间。根据系统基因组分析，这四株菌株聚为一个独立的分支，与噬细胞菌属密切相关。这些菌株的基因组大小约为9.49-9.77 Mbp，DNA G+C含量为38.8-39.0 mol%。它们都含有C ω5c、异-C和异-C 3-OH作为主要脂肪酸，以及甲基萘醌7作为唯一的呼吸醌。它们都以磷脂酰乙醇胺作为主要极性脂质。基于表型和系统基因组特征，这四株菌株应代表一个新属内的两个新物种，为此提出了敏捷黄噬细胞菌新属、新种（BD1B2-1 = GDMCC 1.2890 = JCM 35374）和黄色黄噬细胞菌新种（NT2B1 = GDMCC 1.2889 = JCM 35375）；前者被指定为新属黄噬细胞菌属的模式种。此外，基于表型和系统基因组数据，我们建议将噬细胞菌科中现有的噬细胞菌属重新分类为一个新科——玫瑰红噬细胞菌科新科。这个新科由模式属噬细胞菌属和新属黄噬细胞菌属组成。
