Laborde Milagros R R, Larramendy Marcelo L, Soloneski Sonia
Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nº 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nº 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Mutat Res Genet Toxicol Environ Mutagen. 2023 Oct;891:503682. doi: 10.1016/j.mrgentox.2023.503682. Epub 2023 Aug 18.
Lambda-cyhalothrin (LCT) and its microformulation Karate® (25 % a.i.) were analysed for its genotoxicity and cytotoxicity on Chinese hamster ovary (CHO-K1) cells. Cytokinesis-block micronucleus cytome (CBMN-cyt) and alkaline single-cell gel electrophoresis (SCGE) bioassays were selected to test genotoxicity. Neutral red uptake (NRU), succinic dehydrogenase activity (MTT) and apoptogenic induction were employed for estimating cytotoxicity. Both compounds were analysed within a concentration range of 0.1-100 µg/mL. Only LCT produced a significant augment in the frequency of micronuclei (MNs) when the cultures were exposed to highest concentrations of 10 and 100 µg LCT/mL. A noticeable decrease in NDI was observed for cultures treated with LCT at 10 and 100 µg/mL. Karate® induced the inhibition of both the proportion of viable cells and succinic dehydrogenase activity and triggered apoptosis 24 h of exposition. Whilst an increased GDI in CHO-K1 cells was observed in the treatments with 1-100 µg Karate®/mL, the GDI was not modified in the treatments employing LCT at equivalent doses. SCGE showed that Karate® was more prone to induce genotoxic effects than LCT. Only 50 µg/mL of Karate® was able to increase apoptosis. Our results demonstrate the genomic instability and cytotoxic effects induced by this pyrethroid insecticide, confirming that LCT exposure can result in a severe drawback for the ecological equilibrium of the environment.
分析了高效氯氟氰菊酯（LCT）及其微乳剂功夫®（活性成分含量25%）对中国仓鼠卵巢（CHO-K1）细胞的遗传毒性和细胞毒性。选用胞质分裂阻滞微核细胞组学（CBMN-cyt）和碱性单细胞凝胶电泳（SCGE）生物测定法检测遗传毒性。采用中性红摄取法（NRU）、琥珀酸脱氢酶活性检测法（MTT）和凋亡诱导法评估细胞毒性。两种化合物均在0.1-100μg/mL的浓度范围内进行分析。当培养物暴露于10和100μg LCT/mL的最高浓度时，只有LCT显著增加了微核（MN）频率。在10和100μg/mL的LCT处理组中，观察到核分裂指数（NDI）显著降低。功夫®在暴露24小时后诱导了活细胞比例和琥珀酸脱氢酶活性的抑制，并引发了凋亡。虽然在1-100μg功夫®/mL的处理组中观察到CHO-K1细胞的生长分化指数（GDI）增加，但在同等剂量的LCT处理组中GDI未发生改变。SCGE显示，功夫®比LCT更容易诱导遗传毒性效应。只有50μg/mL的功夫®能够增加细胞凋亡。我们的结果证明了这种拟除虫菊酯类杀虫剂诱导的基因组不稳定性和细胞毒性效应，证实了LCT暴露会对环境的生态平衡造成严重不利影响。
