用于鸡的减毒抗球虫活疫苗。

Live attenuated anticoccidial vaccines for chickens.

机构信息

Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.

National Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of Agriculture and Rural Affairs, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100093, PR China.

出版信息

Trends Parasitol. 2023 Dec;39(12):1087-1099. doi: 10.1016/j.pt.2023.09.002. Epub 2023 Sep 27.

DOI:10.1016/j.pt.2023.09.002

PMID:37770352

Abstract

Chicken coccidiosis, caused by infection with single or multiple Eimeria species, results in significant economic losses to the global poultry industry. Over the past decades, considerable efforts have been made to generate attenuated Eimeria strains, and the use of live attenuated anticoccidial vaccines for disease prevention has achieved tremendous success. In this review, we evaluate the advantages and limitations of the methods of attenuation as well as attenuated Eimeria strains in a historical perspective. Also, we summarize the recent exciting research advances in transient/stable transfection systems and clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing developed for Eimeria parasites, and discuss trends and challenges of developing live attenuated anticoccidial vaccines based on transgenesis and genome editing.

摘要

鸡球虫病是由单一或多种艾美耳属原虫感染引起的，给全球家禽养殖业造成了巨大的经济损失。在过去的几十年中，人们做出了巨大的努力来培育减毒的艾美耳属虫株，使用活的减毒抗球虫疫苗来预防疾病已经取得了巨大的成功。在这篇综述中，我们从历史的角度评估了减毒方法和减毒艾美耳属虫株的优缺点。此外，我们还总结了最近在用于艾美耳属寄生虫的瞬时/稳定转染系统和基于成簇规律间隔短回文重复序列（CRISPR）的基因组编辑方面令人兴奋的研究进展，并讨论了基于转基因和基因组编辑开发活的减毒抗球虫疫苗的趋势和挑战。