Castonguay T W, Dallman M F, Stern J S
Am J Physiol. 1986 Nov;251(5 Pt 2):R923-33. doi: 10.1152/ajpregu.1986.251.5.R923.
Ten-week-old female obese and lean Zucker rats were given access to three separate macronutrient sources (casein, starch, and lard) for 7 days. They were then either adrenalectomized (ADX) or given a sham operation. Rats were assigned to one of three groups and given a daily injection of either 0, 2, or 10 mg of corticosterone. They continued to select a diet for another 17 days, after which they were killed, and their blood was assayed for corticosterone, adrenocorticotropin hormone (ACTH), insulin, glucose, and triglyceride. Retroperitoneal and parametrial fat depots were excised and sampled for lipoprotein lipase activity, fat cell size, and number. Body composition was also determined. Selection patterns of lean and obese rats were markedly affected by both ADX and corticosterone replacement. All three groups of sham-operated obese rats ate significantly more fat than did sham-operated lean rats. Adrenalectomy significantly reduced fat intakes in both obese and lean rats. Corticosterone therapy restored fat appetites of lean and obese rats in a dose-dependent fashion. In comparison to ADX lean rats, ADX obese rats reduced their normally elevated levels of blood glucose, plasma triglycerides, and insulin to within normal limits. Similarly, adipose cellularity of the ADX obese rats was reduced to that of sham-operated lean rats. Carcass fat was significantly reduced after adrenalectomy. Corticosterone therapy prevented the reduction in a dose-dependent way.
给10周龄的雌性肥胖和瘦型 Zucker 大鼠提供三种不同的常量营养素来源（酪蛋白、淀粉和猪油），持续7天。然后对它们进行肾上腺切除术（ADX）或假手术。将大鼠分为三组，每天分别注射0、2或10毫克皮质酮。它们继续选择饮食17天，之后处死大鼠，检测其血液中的皮质酮、促肾上腺皮质激素（ACTH）、胰岛素、葡萄糖和甘油三酯。切除腹膜后和子宫旁脂肪库并取样，检测脂蛋白脂肪酶活性、脂肪细胞大小和数量。还测定了身体成分。ADX和皮质酮替代均显著影响瘦型和肥胖大鼠的选择模式。所有三组假手术的肥胖大鼠比假手术的瘦型大鼠摄入的脂肪显著更多。肾上腺切除术显著降低了肥胖和瘦型大鼠的脂肪摄入量。皮质酮治疗以剂量依赖的方式恢复了瘦型和肥胖大鼠的脂肪食欲。与ADX瘦型大鼠相比，ADX肥胖大鼠将其通常升高的血糖、血浆甘油三酯和胰岛素水平降至正常范围内。同样，ADX肥胖大鼠的脂肪细胞数量减少到假手术瘦型大鼠的水平。肾上腺切除术后胴体脂肪显著减少。皮质酮治疗以剂量依赖的方式阻止了这种减少。
