Antibody-Free Fluorine-Assisted Metabolic Sequencing of RNA -Acetylcytidine.

-Acetylcytidine (acC) has been found to affect a variety of cellular and biological processes. For a mechanistic understanding of the roles of acC in biology and disease, we present an antibody-free, fluorine-assisted metabolic sequencing method to detect RNA acC, called "FAM-seq". We successfully applied FAM-seq to profile acC landscapes in human 293T, HeLa, and MDA cell lines in parallel with the reported acRIP-seq method. By comparison with the classic acC antibody sequencing method, we found that FAM-seq is a convenient and reliable method for transcriptome-wide mapping of acC. Because this method holds promise for detecting nascent RNA acC modifications, we further investigated the role of acC in regulating chemotherapy drug resistance in chronic myeloid leukemia. The results indicated that drug development or combination therapy could be enhanced by appreciating the key role of acC modification in cancer therapy.