衰老及年龄相关性玻璃膜疣猕猴脉络膜变化

Choroidal Changes in Rhesus Macaques in Aging and Age-Related Drusen.

机构信息

Department of Ophthalmology & Vision Science, University of California, Davis, Sacramento, California, United States.

Department of Ophthalmology, California Northstate University, College of Medicine, Elk Grove, California, United States.

出版信息

Invest Ophthalmol Vis Sci. 2023 Sep 1;64(12):44. doi: 10.1167/iovs.64.12.44.

DOI:10.1167/iovs.64.12.44

PMID:37773500

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10547013/

Abstract

PURPOSE

Choroidal vascular changes occur with normal aging and age-related macular degeneration (AMD). Here, we evaluate choroidal thickness and vascularity in aged rhesus macaques to better understand the choroid's role in this nonhuman primate model of AMD.

METHODS

We analyzed optical coherence tomography (OCT) images of 244 eyes from 122 rhesus macaques (aged 4-32 years) to measure choroidal thickness (CT) and choroidal vascularity index (CVI). Drusen number, size, and volume were measured by semiautomated annotation and segmentation of OCT images. We performed regression analyses to determine any association of CT or CVI with age, sex, and axial length and to determine if the presence and volume of soft drusen impacted these choroidal parameters.

RESULTS

In rhesus macaques, subfoveal CT decreased with age at 3.2 µm/y (R2 = 0.481, P < 0.001), while CVI decreased at 0.66% per year (R2 = 0.257, P < 0.001). Eyes with soft drusen exhibited thicker choroid (179.9 ± 17.5 µm vs. 162.0 ± 27.9 µm, P < 0.001) and higher CVI (0.612 ± 0.051 vs. 0.577 ± 0.093, P = 0.005) than age-matched control animals. Neither CT or CVI appeared to be associated with drusen number, size, or volume in this cohort. However, some drusen in macaques were associated with underlying choroidal vessel enlargement resembling pachydrusen in human patients with AMD.

CONCLUSIONS

Changes in the choroidal vasculature in rhesus macaques resemble choroidal changes in human aging, but eyes with drusen exhibit choroidal thickening, increased vascularity, and phenotypic characteristics of pachydrusen observed in some patients with AMD.

摘要

目的

脉络膜血管变化发生于正常衰老和年龄相关性黄斑变性（AMD）。在这里，我们评估了老年恒河猴的脉络膜厚度和血管密度，以更好地了解脉络膜在该非人灵长类 AMD 模型中的作用。

方法

我们分析了 122 只恒河猴（年龄 4-32 岁）的 244 只眼的光学相干断层扫描（OCT）图像，以测量脉络膜厚度（CT）和脉络膜血管指数（CVI）。通过 OCT 图像的半自动注释和分割来测量玻璃膜疣的数量、大小和体积。我们进行了回归分析，以确定 CT 或 CVI 与年龄、性别和眼轴长度的任何关联，并确定软玻璃膜疣的存在和体积是否影响这些脉络膜参数。

结果

在恒河猴中，黄斑中心凹下 CT 随年龄以 3.2 µm/y 的速度下降（R2 = 0.481，P < 0.001），而 CVI 以每年 0.66%的速度下降（R2 = 0.257，P < 0.001）。有软玻璃膜疣的眼睛脉络膜更厚（179.9 ± 17.5 µm 比 162.0 ± 27.9 µm，P < 0.001），CVI 更高（0.612 ± 0.051 比 0.577 ± 0.093，P = 0.005），与年龄匹配的对照组动物相比。在这个队列中，CT 或 CVI 似乎都与玻璃膜疣的数量、大小或体积无关。然而，一些恒河猴的玻璃膜疣与潜在的脉络膜血管扩张有关，类似于 AMD 患者的厚玻璃膜疣。