Department of Biostatistics and Bioinformatics of National Institute of Cardiology, Ministry of Health, Rio de Janeiro, Brazil.
Department of Biostatistics and Bioinformatics of National Institute of Cardiology, Ministry of Health, Rio de Janeiro, Brazil.
Stem Cell Reports. 2023 Oct 10;18(10):1905-1912. doi: 10.1016/j.stemcr.2023.09.001. Epub 2023 Sep 28.
Identifying human leukocyte antigen (HLA) haplotype-homozygous donors for the generation of induced pluripotent stem (iPS) cell lines permits the construction of biobanks immunologically compatible with significant numbers of individuals for use in therapy. However, two questions must be addressed to create such a bank: how many cell lines are necessary to match most of the recipient population and how many people should be tested to find these donors? In Japan and the UK, 50 and 100 distinct HLA-A, -B, and -DRB1 triple-homozygous haplotypes would cover 90% of those populations, respectively. Using data from the Brazilian National Registry of Bone Marrow Donors (REDOME), encompassing 4,017,239 individuals, we identified 1,906 distinct triple-homozygous HLA haplotypes. In Brazil, 559 triple-homozygous cell lines cover 95% of the population, and 3.8 million people would have to be screened. Finally, we show the contribution of the 30 most frequent triple-homozygous HLA haplotypes in Brazil to populations of different countries.
鉴定人类白细胞抗原（HLA）单体型纯合供体，用于生成诱导多能干细胞（iPS）系，可构建与大量个体免疫相容的生物库，用于治疗。但是，要创建这样一个银行，必须解决两个问题：需要多少个细胞系来匹配大多数受者群体，以及需要测试多少人来找到这些供体？在日本和英国，50 和 100 个不同的 HLA-A、-B 和 -DRB1 三重纯合单体型将分别覆盖这些人群的 90%。利用来自巴西骨髓捐赠者国家登记处（REDOME）的数据，涵盖了 4,017,239 个人，我们确定了 1,906 个不同的三重纯合 HLA 单体型。在巴西，559 个三重纯合细胞系覆盖了 95%的人口，需要筛查 380 万人。最后，我们展示了巴西 30 个最常见的三重纯合 HLA 单体型对不同国家人群的贡献。
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