台湾地区不同表型的肠道微生物群分析及 Blastocystis 阴性个体
Intestinal microbiota analysis of different Blastocystis subtypes and Blastocystis-negative individuals in Taiwan.
机构信息
Graduate Institute of Pathology and Parasitology, National Defense Medical Center, Taipei, Taiwan; Department of Inspection, Taipei City Hospital, Renai Branch, Taipei, Taiwan; Host-Parasite Interactions Laboratory, National Defense Medical Center, Taipei, Taiwan.
Genomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
出版信息
Biomed J. 2024 Aug;47(4):100661. doi: 10.1016/j.bj.2023.100661. Epub 2023 Sep 27.
BACKGROUND
Intestinal parasitic infections are the most common infectious diseases among Southeast Asian migrant workers in Taiwan, especially for infections with Blastocystis hominis. However, little is known about the impact of Blastocystis subtypes (STs) on the gut microbiota.
METHODS
We retrospectively evaluated the prevalence of intestinal parasites in a teaching hospital in Northern Taiwan in the period of 2015-2019. Blastocystis-positive stool specimens were collected for ST analysis by polymerase chain reaction in 2020. Intestinal microbiota analyses of different Blastocystis STs and Blastocystis-free individuals were conducted by 16S rRNA sequencing.
RESULTS
A total of 13,859 subjects were analyzed, of which 1802 cases (13%) were diagnosed with intestinal parasitic infections. B. hominis infections were the most prevalent (n = 1546, 85.7%). ST analysis of Blastocystis-positive samples (n = 150) indicated that ST1 was the most common type, followed by ST3, ST4, ST2, ST7, and ST5. Different Blastocystis STs (ST1, ST3, and ST4) were associated with distinct richness and diversity of the microbiota. Taxonomic profiles revealed that Akkermansia muciniphila was significantly enriched for all analyzed Blastocystis STs, whereas Holdemanella biformis was more abundant in the Blastocystis-free group. Additionally, Succinivibrio dextrinosolvens and Coprococcus eutactus were specifically more abundant in ST3 carriers than in non-infected individuals.
CONCLUSION
This study demonstrates that A. muciniphila is positively associated with all Blastocystis STs, while H. biformis was negatively associated with them. Several bacteria were enriched in specific STs, highlighting the need for further microbiota analysis at the ST level to elucidate the pathogenicity of Blastocystis.
背景
肠道寄生虫感染是台湾东南亚移民工人中最常见的传染病，尤其是感染了人芽囊原虫。然而，对于人芽囊原虫的亚型（ST）对肠道微生物群的影响知之甚少。
方法
我们回顾性评估了 2015 年至 2019 年期间台湾北部一所教学医院的肠道寄生虫流行情况。2020 年，收集人芽囊原虫阳性粪便标本，通过聚合酶链反应进行 ST 分析。通过 16S rRNA 测序对不同人芽囊原虫 ST 和无芽囊原虫个体的肠道微生物群进行分析。
结果
共分析了 13859 例患者，其中 1802 例（13%）诊断为肠道寄生虫感染。人芽囊原虫感染最为常见（n=1546，85.7%）。对人芽囊原虫阳性样本（n=150）的 ST 分析表明，ST1 是最常见的类型，其次是 ST3、ST4、ST2、ST7 和 ST5。不同的人芽囊原虫 ST（ST1、ST3 和 ST4）与微生物群的不同丰富度和多样性相关。分类群谱表明，所有分析的人芽囊原虫 ST 均显著富集阿克曼氏菌（Akkermansia muciniphila），而无芽囊原虫组中双歧杆菌（Holdemanella biformis）更为丰富。此外，琥珀酸弧菌（Succinivibrio dextrinosolvens）和粪真杆菌（Coprococcus eutactus）在 ST3 携带者中比非感染者中更为丰富。
结论
本研究表明，阿克曼氏菌与人芽囊原虫的所有 ST 均呈正相关，而双歧杆菌与人芽囊原虫的所有 ST 均呈负相关。一些细菌在特定 ST 中富集，这突出表明需要在 ST 水平进一步进行微生物组分析，以阐明人芽囊原虫的致病性。
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