A. P. Avtsyn Research Institute of Human Morphology, B. V. Pet-rovsky Russian Research Center of Surgery, Moscow, Russia.
Bull Exp Biol Med. 2023 Aug;175(4):513-518. doi: 10.1007/s10517-023-05897-w. Epub 2023 Sep 30.
Structural and functional parameters of mast cells (MC) in rat mammary glands during experimentally provoked breast cancer were studied by the cytogram, index of cell saturation with secretory products, and degranulation index. The cytogram was calculated in histological sections stained with toluidine blue. The functional conditions of MC were determined by electron microscopy. The study revealed expansion of MC population and activation of their functional state evidenced by significant prevalence of cells with high degranulation degree and reduced saturation index. At the ultrastructural level, MC were characterized by deformation, polymorphism, paleness of secretory granules, and elevation of the number of lacunae in the cytoplasm. The study showed that MC are active players in tumor microenvironment. Remembering heteromorphism of MC, further study of their role in pathogenesis of various tumor diseases seems promising.
研究了在实验性诱发乳腺癌过程中大鼠乳腺肥大细胞（MC）的结构和功能参数，使用细胞谱、细胞分泌产物饱和度指数和脱颗粒指数进行研究。细胞谱通过甲苯胺蓝染色的组织学切片进行计算。MC 的功能状态通过电子显微镜确定。研究表明，MC 群体扩张，其功能状态被激活，表现为高度脱颗粒程度的细胞显著增多，而饱和度指数降低。在超微结构水平上，MC 的特征为变形、多形性、分泌颗粒苍白和细胞质中腔隙数量增加。研究表明，MC 是肿瘤微环境中的活跃参与者。鉴于 MC 的异型性，进一步研究它们在各种肿瘤疾病发病机制中的作用似乎很有前途。
