Key Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, China.
Key Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 5):127125. doi: 10.1016/j.ijbiomac.2023.127125. Epub 2023 Sep 28.
Biomaterials with excellent biocompatibility, mechanical performance, and self-recovery properties are urgently needed for tissue regeneration. Inspired by barnacle cement and spider silk, we genetically designed and overexpressed a fused protein (cp19k-MaSp1) composed of Megabalanus rosa (cp19k) and Nephila clavata dragline silk protein (MaSp1) in Pichia pastoris. The recombinant cp19k-MaSp1 exhibited enhanced adhesion capability beyond those of the individual proteins in both aqueous and non-aqueous conditions. cp19k-MaSp1 protein fiber scaffolds prepared through electrospinning have adequate hydrophilicity compared to cp19k and MaSp1 protein fiber scaffolds, and offer improved overall porosity compared to MaSp1 protein fiber scaffolds. The cp19k-MaSp1 protein fiber scaffolds showed excellent proteolytically stable properties because of only 9.6 % depletion after incubation in a biodegradation solution for 56 d. The cp19k-MaSp1 protein fiber scaffolds present remarkably high extreme tensile strength (112.7 ± 11.6 MPa) and superior ductility (438.4 ± 43.9 %) compared with cp19k (34.4 ± 8.1 MPa, 115.4 ± 32.7 %) and MaSp1 protein fiber scaffolds (65.8 ± 9.3 MPa, 409.6 ± 23.1 %), also 68.4 % of tensile strength was recovered by incubation in K buffer after multiple stretches, which create a favorable cell adhesion, growth, and proliferation environment for human umbilical vein endothelial cells (HUVECs). The improved biocompatibility, extensive adhesion, mechanical strength, and self-recovery properties make the bioinspired synthetic cp19k-MaSp1 a potential candidate for biomedical tissue reconstruction.
用于组织再生的生物材料急需具有优异的生物相容性、机械性能和自我恢复性能。受藤壶水泥和蜘蛛丝的启发，我们在毕赤酵母中基因设计并过表达了一种由玫瑰巨蛎（cp19k）和 Nephila clavata 牵引丝蛋白（MaSp1）组成的融合蛋白（cp19k-MaSp1）。重组 cp19k-MaSp1 在水相和非水相条件下均表现出比单个蛋白更强的粘附能力。与 cp19k 和 MaSp1 蛋白纤维支架相比，通过静电纺丝制备的 cp19k-MaSp1 蛋白纤维支架具有足够的亲水性，与 MaSp1 蛋白纤维支架相比，其整体孔隙率得到了提高。由于在生物降解溶液中孵育 56 天后仅消耗 9.6%，cp19k-MaSp1 蛋白纤维支架具有优异的酶解稳定性。与 cp19k（34.4 ± 8.1 MPa，115.4 ± 32.7%）和 MaSp1 蛋白纤维支架（65.8 ± 9.3 MPa，409.6 ± 23.1%）相比，cp19k-MaSp1 蛋白纤维支架具有显著更高的极限拉伸强度（112.7 ± 11.6 MPa）和更好的延展性（438.4 ± 43.9%），经过多次拉伸后在 K 缓冲液中孵育可恢复 68.4%的拉伸强度，这为人脐静脉内皮细胞（HUVECs）创造了有利的细胞粘附、生长和增殖环境。仿生合成 cp19k-MaSp1 的生物相容性、广泛的粘附性、机械强度和自我恢复特性使其成为生物医学组织重建的潜在候选材料。
