Yang Xiaolan, Chen Chaoming, Wang Keyang, Chen Min, Wang Yong, Chen Zhengping, Zhao Wang, Ou Shu
Department of Neurology, The Fengjie People's Hospital, Fengjie Branch of the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Department of Neurology, Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.
Front Genet. 2023 Sep 14;14:1231682. doi: 10.3389/fgene.2023.1231682. eCollection 2023.
Neuropathic pain remains a prevalent and challenging condition to treat, with current therapies often providing inadequate relief. Ozone therapy has emerged as a promising treatment option; however, its mechanisms of action in neuropathic pain remain poorly understood. In this study, we investigated the effects of ozone treatment on gene expression and metabolite levels in the brainstem and hypothalamus of a rat model, using a combined transcriptomic and metabolomic approach. Our findings revealed significant alterations in key genes, including DCST1 and AIF1L, and metabolites such as Aconitic acid, L-Glutamic acid, UDP-glucose, and Tyrosine. These changes suggest a complex interplay of molecular pathways and region-specific mechanisms underlying the analgesic effects of ozone therapy. Our study provides insights into the molecular targets of ozone treatment for neuropathic pain, laying the groundwork for future research on validating these targets and developing novel therapeutic strategies.
神经性疼痛仍然是一种普遍且难以治疗的疾病，目前的治疗方法往往无法提供充分的缓解。臭氧疗法已成为一种有前景的治疗选择；然而，其在神经性疼痛中的作用机制仍知之甚少。在本研究中，我们采用转录组学和代谢组学相结合的方法，研究了臭氧治疗对大鼠模型脑干和下丘脑基因表达及代谢物水平的影响。我们的研究结果揭示了关键基因（包括DCST1和AIF1L）以及代谢物（如乌头酸、L-谷氨酸、UDP-葡萄糖和酪氨酸）的显著变化。这些变化表明，分子途径和区域特异性机制之间存在复杂的相互作用，是臭氧疗法镇痛作用的基础。我们的研究为臭氧治疗神经性疼痛的分子靶点提供了见解，为未来验证这些靶点和开发新的治疗策略的研究奠定了基础。
