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提取物革新纳米技术:具有强大抗氧化和抗菌活性的银纳米颗粒的生物合成

Revolutionizing Nanotechnology with Extracts: Biogenic Synthesis of Silver Nanoparticles Exhibiting Potent Antioxidant and Antibacterial Activities.

作者信息

Abida Abida, Almutairi Mikhlid H, Mushtaq Nadia, Ahmed Mushtaq, Sher Naila, Fozia Fozia, Ahmad Ijaz, Almutairi Bader O, Ullah Zia

机构信息

Department of Botany, University of Science and Technology, Bannu 28100, KPK, Pakistan.

Zoology Department, College of Science, King Saud University, P.O. Box: 2455, Riyadh 11451, Saudi Arabia.

出版信息

ACS Omega. 2023 Sep 13;8(38):35140-35151. doi: 10.1021/acsomega.3c04373. eCollection 2023 Sep 26.

Abstract

In this study, we described the environmentally friendly biosynthesis of silver nanoparticles (AgNPs) utilizing ethanolic extract of () as a capping and reducing agent. We also looked at the antioxidant and antibacterial capacities of AgNPs. In order to determine the size, shape, and crystallinity of the created AgNPs, the current project was designed to produce AgNPs utilizing the crude extract of the . The effectiveness of the project was evaluated by UV-visible spectrophotometry, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. AgNPs are monodispersed and spherical and have 50 nm average particle diameters, as determined using Image J software calculations and SEM observation. Four significant peaks from an XRD study, located at 38.46, 44.63, 64.81, and 77.74 nm, were used to calculate the average crystalline size of AgNPs which was reported to be 15 nm. In the crude extract of , it is possible to see the functional group peaks of a number of substances that are essential for bioreduction and the stability of the AgNPs. Antibacterial and antioxidant properties of AgNPs in vitro (DPPH, ABTS, H2O2, phosphomolybdenum, and ferric reducing power) were examined using conventional methods. The AgNPs showed maximum DPPH (72.51% with IC50 = 144.61 μg/mL), ABTS (75.24% with IC50 = 131.21 μg/mL), hydrogen peroxide (73.33% with IC50 = 115.05 μg/mL), phosphomolybdenum activity (73.43% with IC50 = 75.25 μg/mL), and observing reducing power (0.25) at a concentration of 250 g/mL. Inhibition by the AgNPs against the bacterial strain was greatest (12 mm). According to the current findings, AgNPs produced by have the highest potential for free radical scavenging and antibacterial activity, which can result in antioxidant and antibiotic agents.

摘要

在本研究中,我们描述了利用()的乙醇提取物作为封端和还原剂,进行银纳米颗粒(AgNPs)的环境友好型生物合成。我们还研究了AgNPs的抗氧化和抗菌能力。为了确定所制备的AgNPs的尺寸、形状和结晶度,本项目旨在利用()的粗提取物来制备AgNPs。通过紫外可见分光光度法、傅里叶变换红外光谱法、扫描电子显微镜和X射线衍射对该项目的有效性进行了评估。使用Image J软件计算和扫描电子显微镜观察确定,AgNPs呈单分散球形,平均粒径为50 nm。X射线衍射研究中的四个显著峰位于38.46、44.63、64.81和77.74 nm处,用于计算AgNPs的平均晶体尺寸,据报道为15 nm。在()的粗提取物中,可以看到许多对生物还原和AgNPs稳定性至关重要的物质的官能团峰。使用传统方法检测了AgNPs在体外的抗菌和抗氧化性能(DPPH、ABTS、H2O2、磷钼酸和铁还原能力)。在浓度为250 μg/mL时,AgNPs表现出最大的DPPH清除率(72.51%,IC50 = 144.61 μg/mL)、ABTS清除率(75.24%,IC50 = 131.21 μg/mL)、过氧化氢清除率(73.33%,IC50 = 115.05 μg/mL)、磷钼酸活性(73.43%,IC50 = 75.25 μg/mL)和还原能力(0.25)。AgNPs对细菌菌株()的抑制作用最大(12 mm)。根据目前的研究结果,由()制备的AgNPs具有最高的自由基清除和抗菌活性潜力,这可能会产生抗氧化剂和抗生素制剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d965/10536851/21abc2cec5ca/ao3c04373_0002.jpg

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