Withler R E, McPhail J D, Devlin R H
Biochem Genet. 1986 Oct;24(9-10):701-13. doi: 10.1007/BF00499003.
We used single-family crosses to confirm the Mendelian interpretation of allozyme variation and to examine linkage relationships at five polymorphic loci in freshwater and anadromous threespine sticklebacks (Gasterosteus aculeatus) from the Little Campbell River, British Columbia. The electrophoretic pattern of a sixth locus, mitochondrial NADP-dependent isocitrate dehydrogenase (IDH), was found to be sexually dimorphic but otherwise invariant in sticklebacks. This raised the possibility of a sex-linked Idh locus. No differences in IDH quantity or substrate affinities (apparent Km values) were detected between male and female sticklebacks. The electrophoretic pattern of IDH expression was not changed in sticklebacks in which sexual development was altered by hormonal treatment.
我们利用单一家系杂交来证实对同工酶变异的孟德尔式解释，并研究来自不列颠哥伦比亚省小坎贝尔河的淡水和溯河产卵三刺鱼（Gasterosteus aculeatus）中五个多态位点的连锁关系。发现第六个位点——线粒体NADP依赖性异柠檬酸脱氢酶（IDH）的电泳图谱在三刺鱼中具有性别二态性，但在其他方面不变。这增加了存在一个与性别相关的Idh位点的可能性。在雄性和雌性三刺鱼之间未检测到IDH数量或底物亲和力（表观Km值）的差异。在通过激素处理改变了性发育的三刺鱼中，IDH表达的电泳图谱没有变化。
