Marimuthu Sankarapandian, Puvaneswari Subramanian, Lakshmanan Ramamoorthy
Department of Zoology, Annamalai University, Chidambaram, 608002, Tamil Nadu, India.
Department of Zoology, Dharmapuram Gnanambigai Govt. Arts College for Women, Mayiladuthurai, 609001, Tamil Nadu, India.
Appl Biochem Biotechnol. 2024 Jul;196(7):3860-3890. doi: 10.1007/s12010-023-04729-x. Epub 2023 Oct 4.
The use of probiotics in shrimp farms has expanded as an alternative to antibiotics, improving shrimp health, growth, disease control and water quality. However, the efficacy of probiotics in intensive systems using biofloc remains uncertain. This study investigated bioremediation and biocontrol of commercial probiotics using biofloc, analysing water quality and bacterial groups influencing shrimp performance. Thirteen microbial colonies were observed in the biofloc samples, and their evolutionary history was inferred using the neighbour-joining method. Analysis using MEGA6 software revealed 99.6% similarity between colony 1P1 and Virgibacillus sp. and 99.8% similarity between colony 2P2 and Bacillus kochii. The study analysed the biochemical and amino acid content of shrimp cultured using biofloc technology for 85 days. The study found that biofloc-cultured L. Vannamei flesh had higher levels of moisture (31%), ash (15.2%), protein (34%) and lipid (12.6%). No seasonal variations were observed between biofloc samples in winter and summer seasons. Protein levels were found to be appropriate for shrimp growth in both ex situ and in situ biofloc systems. The average growth rate (kg/m) of biofloc-cultured shrimp was significantly greater than in normal pond shrimp in both samples. The feed conversion ratio in the biofloc tank was lower than in the usual pond-cultured shrimp, possibly due to lower growth rate, lower feeding frequency (2 times per day) and higher density. The growth of L. vannamei shrimp is influenced by stocking density, with higher stocking density affecting the average weight. The study also examined the growth biochemical and amino acid content of shrimp cultured using biofloc technology.
益生菌在对虾养殖场的使用已有所扩大，作为抗生素的替代品，可改善对虾健康、生长、疾病控制及水质。然而，在使用生物絮团的集约化养殖系统中，益生菌的功效仍不确定。本研究调查了使用生物絮团的商业益生菌的生物修复和生物控制作用，分析了影响对虾生长性能的水质和细菌群落。在生物絮团样本中观察到13个微生物菌落，并使用邻接法推断它们的进化历史。使用MEGA6软件分析发现，菌落1P1与 Virgibacillus sp. 的相似度为99.6%，菌落2P2与Bacillus kochii的相似度为99.8%。该研究分析了使用生物絮团技术养殖85天的对虾的生化和氨基酸含量。研究发现，生物絮团养殖的凡纳滨对虾虾肉的水分含量较高（31%）、灰分含量较高（15.2%）、蛋白质含量较高（34%）和脂质含量较高（12.6%）。在冬季和夏季的生物絮团样本之间未观察到季节性变化。发现在异地和原位生物絮团系统中，蛋白质水平都适合对虾生长。在两个样本中，生物絮团养殖对虾的平均生长率（kg/m）均显著高于正常池塘养殖的对虾。生物絮团养殖池中的饲料转化率低于常规池塘养殖的对虾，这可能是由于生长率较低、投喂频率较低（每天2次）和密度较高所致。凡纳滨对虾的生长受放养密度影响，放养密度越高，平均体重越低。该研究还检测了使用生物絮团技术养殖的对虾的生长生化和氨基酸含量。
