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巴西中西部一高内脏利什曼病发病率地区自然感染内脏利什曼原虫的长角血蝉。

Natural infection of Lutzomyia longipalpis (Lutz & Neiva, 1912) by Leishmania infantum in a municipality with a high incidence of visceral leishmaniasis in the Brazilian Midwest.

机构信息

Universidade Federal da Grande Dourados, Programa de Pós-graduação em Ciências da Saúde, Dourados, MS, Brasil.

Secretaria Municipal de Saúde, Três Lagoas, MS, Brasil.

出版信息

Rev Soc Bras Med Trop. 2023 Sep 22;56:e02592023. doi: 10.1590/0037-8682-0259-2023. eCollection 2023.

Abstract

BACKGROUND

Here, Leishmania presence in sand flies from Três Lagoas, Mato Grosso do Sul, Brazil, after visceral leishmaniasis (VL) was investigated.

METHODS

In April 2022, two light traps were deployed within and around the residence for two days post-VL case report.

RESULTS

A total of 120 Lutzomyia longipalpis were collected. Suprapyloric flagellates were found in a female sand fly with eggs and residual blood during midgut dissection. Sequencing of ITS1 and cytb fragments confirmed Leishmania infantum DNA and identified Homo sapiens as the blood source, respectively.

CONCLUSIONS

This study emphasizes the importance of monitoring sand flies in VL endemic areas.

摘要

背景

本研究调查了巴西南马托格罗索州特雷斯莱奥达斯市内脏利什曼病(VL)病例后,在该地的沙蝇中是否存在利什曼原虫。

方法

2022 年 4 月,在 VL 病例报告后,在住所内及其周围放置了两个诱捕器,持续两天。

结果

共收集到 120 只长角血蝉。在进行中肠解剖时,一只带有卵和残余血液的雌性沙蝇中发现了胃上鞭毛原虫。ITS1 和 cytb 片段的测序分别证实了利什曼原虫婴儿亚种 DNA,并确定人类为血液来源。

结论

本研究强调了在 VL 流行地区监测沙蝇的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4600/10550212/4f501451e39c/1678-9849-rsbmt-56-e0259-2023-gf1.jpg

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