Woody C D, Bartfai T, Gruen E, Nairn A C
Brain Res. 1986 Oct 29;386(1-2):379-85. doi: 10.1016/0006-8993(86)90175-7.
Purified, cyclic GMP-dependent protein kinase (cGPK) was pressure-injected into neurons of the precruciate cortex of awake cats. Input resistances increased within seconds after injection and remained elevated for 2 min or longer. The increases were larger when cGPK was injected in a mixture with 10 microM cGMP than when injected alone. Injections of heat-inactivated cGPK, with or without 10 microM cGMP, failed to produce increases in input resistance. The present results indicate that injection of activated cGPK into neurons of the mammalian motor cortex can mimic actions of extracellularly applied acetylcholine and intracellularly applied cGMP, the latter in 100-fold higher concentrations than those used here, in neurons of the same cortical areas.
将纯化的环磷酸鸟苷依赖性蛋白激酶（cGPK）压力注射到清醒猫的前十字皮质神经元中。注射后数秒内输入电阻增加，并持续升高2分钟或更长时间。当cGPK与10微摩尔环磷酸鸟苷（cGMP）混合注射时，电阻增加幅度比单独注射时更大。注射热灭活的cGPK（无论有无10微摩尔cGMP）均未能使输入电阻增加。目前的结果表明，将活化的cGPK注射到哺乳动物运动皮质神经元中，可以模拟细胞外施加乙酰胆碱和细胞内施加cGMP（后者浓度比此处所用浓度高100倍）在相同皮质区域神经元中的作用。
