Kurobe Tomofumi, Kurita Jun, Haenen Olga, Voorbergen-Laarman Michal, Ito Takafumi
Pathology Division, Nansei Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, Minami-Ise, Mie, Japan.
Pathology Division, Tamaki Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, Tamaki, Mie, Japan.
J Fish Dis. 2024 Jan;47(1):e13868. doi: 10.1111/jfd.13868. Epub 2023 Oct 5.
In 2011 and 2015, four mass mortalities of Prussian carp (Carassius gibelio) were observed in a recreational freshwater lake and open freshwater in the western part of the Netherlands. Cyprinid herpesvirus 2 (CyHV-2) infection was suspected in these cases, based on presumptive gross diagnosis. To elucidate the cause of the mass mortalities diagnostic PCR assays were performed for CyHV-2, based on the helicase gene. Furthermore, the viral isolates were genotyped by sequencing the enlarged marker A and marker B sequences. Diagnostic PCR revealed that three of four samples were positive for CyHV-2, indicating these three mass mortalities were associated with CyHV-2 infection. The marker A sequence from one of the isolates found in this study was identical to those from different locations such as Asia and Middle East, suggesting a link among the isolates. This is the first detailed report on mass mortalities of Prussian carp associated with CyHV-2 infection in natural aquatic environments in the Netherlands. Since 2015, additionally, in total three CyHV-2 associated outbreaks of Dutch Prussian carp were seen in 2016 and 2020. These outbreaks in Prussian carp from lakes and open water suggest that the virus has been spreading in natural freshwaters in the Netherlands.
2011年和2015年，在荷兰西部的一个休闲淡水湖和开放淡水中观察到四次欧洲鲫（银鲫）大规模死亡事件。基于初步的大体诊断，怀疑这些病例是由鲤疱疹病毒2型（CyHV-2）感染所致。为了阐明大规模死亡的原因，基于解旋酶基因对CyHV-2进行了诊断性聚合酶链反应（PCR）检测。此外，通过对扩增的标记A和标记B序列进行测序，对病毒分离株进行了基因分型。诊断性PCR检测显示，四个样本中有三个对CyHV-2呈阳性，表明这三次大规模死亡与CyHV-2感染有关。本研究中发现的一个分离株的标记A序列与来自亚洲和中东等不同地区的序列相同，这表明这些分离株之间存在联系。这是关于荷兰自然水生环境中与CyHV-2感染相关的欧洲鲫大规模死亡的第一份详细报告。此外，自2015年以来，2016年和2020年总共又出现了三次与CyHV-2相关的荷兰欧洲鲫疫情。这些来自湖泊和开放水域的欧洲鲫疫情表明，该病毒一直在荷兰的天然淡水中传播。
