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毛发中乙基葡萄糖醛酸苷的测定与大学生自述饮酒量的关系。

Determination of ethyl glucuronide in hair and self-reported alcohol consumption in university students.

机构信息

Department of Medical and Surgical Sciences, Unit of Legal Medicine, University of Bologna, via Irnerio 49, Bologna, Italy.

Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, Italy.

出版信息

Forensic Sci Med Pathol. 2024 Sep;20(3):769-777. doi: 10.1007/s12024-023-00727-x. Epub 2023 Oct 5.

DOI:10.1007/s12024-023-00727-x
PMID:37796377
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11525380/
Abstract

Young individuals constitute an intriguing population, as their drinking habits are notably shaped by their perception of their peers' alcohol consumption. Nonetheless, excessive alcohol intake can have detrimental effects on academic performance, interpersonal relationships, and the risk and severity of accidents. This study reported the first data involving students enrolled from three universities on a voluntary basis for alcohol consumption evaluation. Alcohol consumption was assessed through questionnaires and EtG quantification in hair (hEtG) carried out by liquid chromatography-mass spectrometry (LC-MS/MS) analysis after a solid-phase extraction (SPE) purification step. The results of our study demonstrated that 77.1% of samples tested negative for hEtG or displayed hEtG ≤ 5 pg/mg. Particularly, the student population was not characterized by samples with hEtG indicative of chronic excessive consumption (hEtG ≥ 30 pg/mg). No significant association was identified between biological sex, among the degree course/the year attended, nor in relation to BMI or smoking/coffee consumption. Among the obtained results, it was worth noting that the comparison of self-reporting abstinence from tobacco and coffee accounted for 65.3% and 16.7%, respectively, while only 2.8% of the total declared abstinence from alcohol. The current study has uncovered a significant level of interest among students in this analysis and its interpretation. This suggests that implementing public health promotion activities within a university setting could be beneficial.

摘要

年轻人是一个有趣的群体,他们的饮酒习惯很大程度上受到同龄人饮酒行为的影响。然而,过量饮酒会对学习成绩、人际关系以及事故风险和严重程度产生负面影响。本研究报告了第一项涉及三所大学学生自愿参与的酒精消费评估数据。通过问卷调查和基于液相色谱-质谱联用(LC-MS/MS)分析的头发中乙醇(EtG)定量(hEtG)评估酒精摄入量,在固相萃取(SPE)净化步骤后进行。研究结果表明,77.1%的样本 hEtG 检测结果为阴性或 hEtG≤5pg/mg。特别是,学生群体中没有 hEtG 指示慢性过量饮酒(hEtG≥30pg/mg)的样本。生物性别、所学专业/年级、BMI 或吸烟/咖啡消费之间均无显著关联。在获得的结果中,值得注意的是,自我报告的戒烟和戒咖啡比例分别为 65.3%和 16.7%,而总戒酒比例仅为 2.8%。目前的研究揭示了学生对这种分析及其解释的浓厚兴趣。这表明在大学环境中实施公共卫生促进活动可能是有益的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfdf/11525380/6ce73d6e898c/12024_2023_727_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfdf/11525380/28c0477fab1a/12024_2023_727_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfdf/11525380/6ce73d6e898c/12024_2023_727_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfdf/11525380/28c0477fab1a/12024_2023_727_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfdf/11525380/6ce73d6e898c/12024_2023_727_Fig2_HTML.jpg

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本文引用的文献

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Alcohol consumption assessment in a student population through combined hair analysis for ethyl glucuronide and fatty acid ethyl esters.通过对乙基葡萄糖醛酸和脂肪酸乙酯进行联合毛发分析来评估学生群体中的酒精消费情况。
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Variability on ethyl glucuronide concentrations in hair depending on sample pretreatment, using a new developed GC-MS/MS method.基于新开发的 GC-MS/MS 方法,发现在毛发中乙基葡萄糖醛酸苷浓度的变化取决于样品预处理。
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Influence of Body Mass Index on Hair Ethyl Glucuronide Concentrations.体重指数对毛发中葡萄糖醛酸乙酯浓度的影响。
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Alcohol, caffeine, and nicotine consumption in adolescents: hair analysis versus self-report.青少年酒精、咖啡因和尼古丁摄入量:毛发分析与自我报告对比
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