Bhupat and Jyoti Mehta School of Biosciences, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, India.
Bhupat and Jyoti Mehta School of Biosciences, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2023 Nov;231:113572. doi: 10.1016/j.colsurfb.2023.113572. Epub 2023 Sep 28.
Pickering emulsion polymerization, stabilized by inorganic nanoparticles such as iron oxide nanoparticles (IONPs), can be used to fabricate scaffolds with the desired porosity and pore size. These nanoparticles create stable emulsions that can be processed under harsh polymerization conditions. IONPs, apart from serving as an emulsifier, impart beneficial bioactivities such as antibacterial and pro-angiogenic activity. Here, we coated IONPs with three different weights of oleic acid (5.0 g, 7.5 g, and 10.0 g) to synthesize oleic acid-IONPs (OA-IONPs) that possess the desired hydrophobicity (contact angle > 100°). Next, glycidyl methacrylate and trimethylolpropane triacrylate were polymerized using the Pickering emulsion polymerization technique stabilized by the OA-IONPs. The physicochemical properties of the resulting porous scaffolds were thoroughly characterized using scanning electron microscopy (SEM), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometry (VSM), and a universal testing machine (UTM). The SEM images confirmed the formation of a porous scaffold. The IONPs content, measured using inductively coupled plasma mass spectrometry (ICP-MS), was in the range of 22-26 µg/mg of the scaffold. The mechanical strengths of the scaffolds were in the range of cancellous bone. The degradation profile of the scaffolds varied between 29% and 41% degradation over 30 days. In vitro cytotoxicity studies conducted using the fibroblast (L929) and osteosarcoma (MG-63) cell lines proved that these scaffolds were non-toxic. SEM images showed that the MG-63 cells adhered firmly to the scaffolds and exhibited a well-spread morphology. The antibacterial activity was confirmed by percentage inhibition studies, SEM analysis of bacterial membrane distortion, and reactive oxygen species (ROS) generation in the bacteria. Chick chorioallantoic membrane assay showed that the total vessel length and branch points were significantly increased in the presence of the scaffolds. These results confirm the pro-angiogenic potential of the fabricated scaffolds. The physicochemical, mechanical, and biological properties of the material suggest that the developed scaffolds would be suitable for bone tissue engineering applications.
Pickering 乳液聚合，由氧化铁纳米粒子（IONP）等无机纳米粒子稳定，可以用来制造具有所需孔隙率和孔径的支架。这些纳米粒子可以形成稳定的乳液，在苛刻的聚合条件下进行处理。IONP 除了作为乳化剂外，还赋予了有益的生物活性，如抗菌和促血管生成活性。在这里，我们用三种不同重量的油酸（5.0g、7.5g 和 10.0g）对 IONP 进行了涂层，以合成具有所需疏水性（接触角>100°）的油酸-IONP（OA-IONP）。然后，使用 Pickering 乳液聚合技术，由 OA-IONP 稳定，聚合甲基丙烯酸缩水甘油酯和三羟甲基丙烷三丙烯酸酯。使用扫描电子显微镜（SEM）、热重分析（TGA）、傅里叶变换红外光谱（FT-IR）、振动样品磁强计（VSM）和万能试验机（UTM）对所得多孔支架的物理化学性质进行了全面表征。SEM 图像证实了多孔支架的形成。使用电感耦合等离子体质谱（ICP-MS）测量的 IONP 含量在支架的 22-26μg/mg 范围内。支架的机械强度在松质骨范围内。支架的降解曲线在 30 天内降解 29%-41%。使用成纤维细胞（L929）和骨肉瘤（MG-63）细胞系进行的体外细胞毒性研究证明，这些支架无毒。SEM 图像显示，MG-63 细胞牢固地附着在支架上，并呈现出良好的伸展形态。通过抑菌率研究、细菌细胞膜变形的 SEM 分析和细菌中活性氧（ROS）的产生证实了抗菌活性。鸡胚绒毛尿囊膜试验表明，在支架存在的情况下，总血管长度和分支点显著增加。这些结果证实了所制备的支架具有促血管生成潜力。材料的物理化学、机械和生物学性质表明，开发的支架将适用于骨组织工程应用。
Colloids Surf B Biointerfaces. 2023-11
J Biomater Sci Polym Ed. 2022-8
J Tissue Eng Regen Med. 2021-4
Biomed Mater. 2022-9-30
Int J Nanomedicine. 2021
Pharmaceutics. 2024-8-21
Comb Chem High Throughput Screen. 2025
Zotero 插件