Bioinspired Device and Tissue Engineering Research Group, School of Biomedical Engineering and Health Sciences, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai, Malaysia.
Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, Skudai, Malaysia.
J Biomater Sci Polym Ed. 2022 Aug;33(11):1349-1368. doi: 10.1080/09205063.2022.2054544. Epub 2022 Mar 30.
Tissue engineering is a cutting-edge approach for using advanced biomaterials to treat defective bone to get desired clinical results. In bone tissue engineering, the scaffolds must have the desired physicochemical and biomechanical natural properties in order to regenerate complicated defective bone. For the first time, polymeric nanocomposite material was developed using cellulose and co-dispersed nanosystem (FeO/GO) by free radical polymerization to fabricate porous polymeric scaffolds freeze drying. Various characterizations techniques, such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM)/energy dispersive X-ray (EDX), and universal testing machine (UTM) were used to investigate structural, morphological, and mechanical properties. Swelling, biodegradation, and wetting analysis were also performed to evaluate their physicochemical behavior. Intercalation of FeO nanoparticles into GO-sheets promoted their dispersion into the polymeric matrix. All porous scaffolds possessed a well-interconnected porous structure, while the synergistic effect of FeO/GO reinforces the mechanical strength of porous scaffolds. The compressive strength and Young's modulus were increased by increasing FeO amount, and maximum mechanical strength was found in HFG-4 and least in HFG-1. However, these porous scaffolds have different swelling and biodegradation behavior due to the variable FeO intercalations into GO-sheets. Antibacterial activities of porous scaffolds were studied against severe Gram-positive and Gram-negative pathogens and increased FeO amount in nanosystem increased the antibacterial activities. The cell viability and morphology of pre-osteoblast () cell lines were studied against porous scaffolds and increased cell viability and proliferation were observed from HFG-1 to HFG-4. Hence, the electroactive material could be the potential material for bone tissue engineering.
组织工程学是一种使用先进生物材料治疗有缺陷骨骼以获得预期临床结果的前沿方法。在骨组织工程中，支架必须具有所需的物理化学和生物力学天然特性，才能再生复杂的缺陷骨骼。首次通过自由基聚合，使用纤维素和共分散纳米系统（FeO/GO）开发了聚合纳米复合材料，以制造多孔聚合支架并通过冷冻干燥进行制造。使用各种特性分析技术，如傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）/能量色散 X 射线（EDX）和万能试验机（UTM）来研究结构、形态和机械性能。还进行了溶胀、生物降解和润湿分析，以评估它们的物理化学行为。FeO 纳米粒子的插层进入 GO 片层中促进了它们在聚合物基质中的分散。所有多孔支架都具有良好的互连多孔结构，而 FeO/GO 的协同作用增强了多孔支架的机械强度。随着 FeO 量的增加，压缩强度和杨氏模量增加，在 HFG-4 中发现最大机械强度，在 HFG-1 中发现最小机械强度。然而，由于 FeO 插入 GO 片层的变量，这些多孔支架具有不同的溶胀和生物降解行为。研究了多孔支架对严重革兰氏阳性和革兰氏阴性病原体的抗菌活性，并且纳米系统中 FeO 量的增加提高了抗菌活性。研究了多孔支架对成骨前体细胞（）细胞系的细胞活力和形态的影响，并且从 HFG-1 到 HFG-4 观察到细胞活力和增殖增加。因此，电活性材料可能是骨组织工程的潜在材料。
