Su Jimmy Jiun-Ming, Lin Chih-Hsin, Chen Hsuan, Lee Shyh-Yuan, Lin Yuan-Min
Institute of Oral Biology, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 110, Taiwan.
Polymers (Basel). 2021 Jul 18;13(14):2354. doi: 10.3390/polym13142354.
Gelatin methacryloyl (GelMA) hydrogel is a photopolymerizable biomaterial widely used for three-dimensional (3D) cell culture due to its high biocompatibility. However, the drawback of GelMA hydrogel is its poor mechanical properties, which may compromise the feasibility of biofabrication techniques. In this study, a cell-laden GelMA composite hydrogel with a combination incorporating silanized hydroxyapatite (Si-HAp) and a simple and harmless visible light crosslinking system for this hydrogel were developed. The incorporation of Si-HAp into the GelMA hydrogel enhanced the mechanical properties of the composite hydrogel. Moreover, the composite hydrogel exhibited low cytotoxicity and promoted the osteogenic gene expression of embedded MG63 cells and Human bone marrow mesenchymal stem cells (hBMSCs). We also established a maskless lithographic method to fabricate a defined 3D structure under visible light by using a digital light processing projector, and the incorporation of Si-HAp increased the resolution of photolithographic hydrogels. The GelMA-Si-HAp composite hydrogel system can serve as an effective biomaterial in bone regeneration.
甲基丙烯酰化明胶（GelMA）水凝胶是一种可光聚合的生物材料，因其具有高生物相容性而被广泛用于三维（3D）细胞培养。然而，GelMA水凝胶的缺点是其机械性能较差，这可能会影响生物制造技术的可行性。在本研究中，开发了一种负载细胞的GelMA复合水凝胶，其包含硅烷化羟基磷灰石（Si-HAp）以及用于该水凝胶的简单且无害的可见光交联系统。将Si-HAp掺入GelMA水凝胶中增强了复合水凝胶的机械性能。此外，复合水凝胶表现出低细胞毒性，并促进了嵌入的MG63细胞和人骨髓间充质干细胞（hBMSCs）的成骨基因表达。我们还建立了一种无掩膜光刻方法，通过使用数字光处理投影仪在可见光下制造确定的3D结构，并且掺入Si-HAp提高了光刻水凝胶的分辨率。GelMA-Si-HAp复合水凝胶系统可作为骨再生中的有效生物材料。
